Neuropeptide y and neuropeptide y Y5 receptor interaction restores impaired growth potential of aging bone marrow stromal cells

Koichi Igura, Husnain K.H. Haider, Rafeeq P.H. Ahmed, Sulaiman Sheriff, Muhammad Ashraf

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Physiological aging impairs the proliferative potential of bone marrow-derived stromal cells. We report here the pro-proliferative response and improved growth characteristics of the aging bone marrow cells subsequent to neuropeptide Y (NPY)/neuropeptide Y Y5 receptor (NPY Y5R) ligand-receptor interaction. Bone marrow cells were isolated from neonatal (2-3 weeks), young (8-12 weeks), and old (24-28 months) rats on the basis of their preferential adherence to plastic surface. After culturing the cells at initial seeding density of 1×10 4 cells/cm 2, we found that the proliferation potential of bone marrow cells declined with age. Real-time polymerase chain reaction (PCR) and Western blotting showed that bone marrow cells in different age groups constitutively expressed NPY and NPY receptor subtypes (Y1R, Y2R, and Y5R). However, NPY and Y5R expression increased by more than 130-fold and decreased by 28-fold, respectively, in old bone marrow cells as compared to young bone marrow cells. NPY (10nM) stimulated the proliferation of all bone marrow cells age groups, and their proliferation was blocked by Y5R antagonist. However, the pro-proliferative effect of NPY on old bone marrow cells was weaker than other cell groups due to lower Y5R expression. Y5R gene transfection of old bone marrow cells with subsequent NPY 3-36 (10nM) treatment significantly increased proliferation of old bone marrow cells (>56%) as compared to green fluorescence protein-transfected control old bone marrow cells. Stimulation of old bone marrow cells by NPY treatment rejuvenated the growth characteristics of aging bone marrow cells as a result of Y5R overexpression.

Original languageEnglish (US)
Pages (from-to)393-403
Number of pages11
JournalRejuvenation Research
Volume14
Issue number4
DOIs
StatePublished - Aug 1 2011
Externally publishedYes

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Neuropeptides
Mesenchymal Stromal Cells
Bone Marrow Cells
Neuropeptide Y
Growth
Neuropeptide Y Receptors
neuropeptide Y5 receptor
Age Groups
Plastics
Transfection
Real-Time Polymerase Chain Reaction
Fluorescence
Western Blotting
Ligands

ASJC Scopus subject areas

  • Aging
  • Geriatrics and Gerontology

Cite this

Neuropeptide y and neuropeptide y Y5 receptor interaction restores impaired growth potential of aging bone marrow stromal cells. / Igura, Koichi; Haider, Husnain K.H.; Ahmed, Rafeeq P.H.; Sheriff, Sulaiman; Ashraf, Muhammad.

In: Rejuvenation Research, Vol. 14, No. 4, 01.08.2011, p. 393-403.

Research output: Contribution to journalArticle

Igura, Koichi ; Haider, Husnain K.H. ; Ahmed, Rafeeq P.H. ; Sheriff, Sulaiman ; Ashraf, Muhammad. / Neuropeptide y and neuropeptide y Y5 receptor interaction restores impaired growth potential of aging bone marrow stromal cells. In: Rejuvenation Research. 2011 ; Vol. 14, No. 4. pp. 393-403.
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