New reagents for determination of amino acids by liquid chromatography with pre-column fluorescence derivatization

Xinjun Fan, Jinmao You, Jingwu Kang, Qingyu Ou, Qingcun Zhu

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

A liquid-chromatography method for the determination of amino acids with fluorescence detection has been developed. The reactions of three fluorescent tagging reagents, namely acridone-N-acetyl chloride (ARC-Cl), carbazole-9-acetyl chloride (CRA-Cl) and carbazole-9-propionyl chloride (CRP-Cl), with amino acids are reported. Emission maxima for the ARC, CRA and CRP derivatives of amino acids were at 430nm (λ(ex)=404nm), 360nm (λ(ex)=335nm) and 365nm (λ(ex)=340nm), respectively. In all cases, the derivatives exhibited strong fluorescence, whereas the reagents themselves also exhibited fluorescence. The labeled derivatives are very stable; <4% decomposition occurs after heating at 40°C for 24h. The fluorescence intensities of the amino-acid derivatives were higher in neutral and alkaline than in acidic solutions. This method, combining a multi-gradient program, offers baseline resolution of the common ARC, CRA and CRP amino-acid derivatives than a linear acetonitrile gradient. Separation of amino-acid derivatives was carried out on a reversed-phase C18 column. Derivatization and chromatographic conditions were optimized. The separation of amino-acid derivatives has good reproducibility and the relative standard deviations (n=6) for 5pmol of each amino acid are <4%. The detection limit is at the fmol level. Copyright (C) 1998 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)81-91
Number of pages11
JournalAnalytica Chimica Acta
Volume367
Issue number1-3
DOIs
StatePublished - Jul 3 1998

Fingerprint

Liquid chromatography
Liquid Chromatography
liquid chromatography
fluorescence
amino acid
Fluorescence
Amino Acids
Derivatives
AIDS-Related Complex
chloride
tagging
Heating
Limit of Detection
decomposition
heating
Decomposition

Keywords

  • Acridone-N-acetyl chloride
  • Amino acids
  • Carbazole-9-acetyl chloride
  • Carbazole-9-yl-propionyl chloride
  • Fluorescence derivatization
  • Liquid chromatography

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy

Cite this

New reagents for determination of amino acids by liquid chromatography with pre-column fluorescence derivatization. / Fan, Xinjun; You, Jinmao; Kang, Jingwu; Ou, Qingyu; Zhu, Qingcun.

In: Analytica Chimica Acta, Vol. 367, No. 1-3, 03.07.1998, p. 81-91.

Research output: Contribution to journalArticle

Fan, Xinjun ; You, Jinmao ; Kang, Jingwu ; Ou, Qingyu ; Zhu, Qingcun. / New reagents for determination of amino acids by liquid chromatography with pre-column fluorescence derivatization. In: Analytica Chimica Acta. 1998 ; Vol. 367, No. 1-3. pp. 81-91.
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