NMR solution structure of attractin, a water-borne protein pheromone from the mollusk Aplysia californica

Ravindranath Garimella, Yuan Xu, Catherine H. Schein, Krishna Rajarathnam, Gregg T. Nagle, Sherry D. Painter, Werner Braun

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Water-borne protein pheromones are essential for coordination of reproductive activities in many marine organisms. In this paper, we describe the first structure of a pheromone protein from a marine organism, that of attractin (58 residues) from Aplysia californica. The NMR solution structure was determined from TOCSY, NOESY, and DQF-COSY measurements of recombinant attractin expressed in insect cells. The sequential resonance assignments were done with standard manual procedures. Approximately 90% of the 949 unambiguous NOESY cross-peaks were assigned automatically with simultaneous three-dimensional structure calculation using our NOAH/DIAMOD/FANTOM program suite. The final bundle of energy-refined structures is well-defined, with an average rmsd value to the mean structure of 0.72 ± 0.12 A for backbone and 1.32 ± 0.11 A for heavy atoms for amino acids 3-47. Attractin contains two antiparallel helices, made up of residues Ile9-Gln16 and I30-S36. The NMR distance constraints are consistent with the three disulfide bonds determined by mass spectroscopy (C4-C41, C13-C33, and C20-C26), where the first two could be directly determined from NOESY cross-peaks between CHβ protons of the corresponding cysteines. The second helix contains the (L/I) 29IEECKTS36 sequence conserved in attractins from five species of Aplysia that could interact with the receptor. The sequence and structure of this region are similar to those of the recognition helix of the Er-11 pheromone of the unicellular ciliate Euplotes raikovi, suggesting a possible common pathway for intercellular communication of these two distinct pheromone families.

Original languageEnglish (US)
Pages (from-to)9970-9979
Number of pages10
JournalBiochemistry
Volume42
Issue number33
DOIs
StatePublished - Aug 26 2003
Externally publishedYes

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Aplysia
Mollusca
Pheromones
Nuclear magnetic resonance
Aquatic Organisms
Water
Proteins
Euplotes
Conserved Sequence
Disulfides
Cysteine
Insects
Protons
Mass Spectrometry
Spectroscopy
Amino Acids
Atoms
Communication

ASJC Scopus subject areas

  • Biochemistry

Cite this

Garimella, R., Xu, Y., Schein, C. H., Rajarathnam, K., Nagle, G. T., Painter, S. D., & Braun, W. (2003). NMR solution structure of attractin, a water-borne protein pheromone from the mollusk Aplysia californica. Biochemistry, 42(33), 9970-9979. https://doi.org/10.1021/bi0274322

NMR solution structure of attractin, a water-borne protein pheromone from the mollusk Aplysia californica. / Garimella, Ravindranath; Xu, Yuan; Schein, Catherine H.; Rajarathnam, Krishna; Nagle, Gregg T.; Painter, Sherry D.; Braun, Werner.

In: Biochemistry, Vol. 42, No. 33, 26.08.2003, p. 9970-9979.

Research output: Contribution to journalArticle

Garimella, R, Xu, Y, Schein, CH, Rajarathnam, K, Nagle, GT, Painter, SD & Braun, W 2003, 'NMR solution structure of attractin, a water-borne protein pheromone from the mollusk Aplysia californica', Biochemistry, vol. 42, no. 33, pp. 9970-9979. https://doi.org/10.1021/bi0274322
Garimella, Ravindranath ; Xu, Yuan ; Schein, Catherine H. ; Rajarathnam, Krishna ; Nagle, Gregg T. ; Painter, Sherry D. ; Braun, Werner. / NMR solution structure of attractin, a water-borne protein pheromone from the mollusk Aplysia californica. In: Biochemistry. 2003 ; Vol. 42, No. 33. pp. 9970-9979.
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