Non-cytotoxic blocking antibodies and suppressor cells induced by donor-specific transfusions in healthy volunteers and potential kidney transplant recipients

Anatolij Horuzsko, Éva Gyódi, Mariann Réti, Klára Ónody, Ferenc Perner, Miklós Kassay, GyózóG G. Petrányi

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11 Scopus citations

Abstract

Donor-specific transfusion (DST)-induced immunosuppression plays a significant role in clinical and experimental transplantation. To clarify the mechanism of suppression on alloreactivity the suppressor cell induction and the non-cytotoxic blocking antibody production and importance was studied in 15 healthy volunteers and 3 kidney transplant recipients (KTR) after DST on mixed lymphocyte culture (MLC). Significant decrease of anti-donor MLC response was found in all of KTR and in 12 cases of the 15 transfused volunteers. Of 18 cases, 15 had blocking factors in their post-DST serum which strongly (52-91%) inhibited the MLC response. The IgG fractions isolated from the "blocking sera" were responsible for this inhibition. Eighty-one percent of non-cytotoxic blocking antibodies affected the responder cells in MLC and reacted with third party responder cells as well. Both buffy coat and platelet transfusions evoked production of the non-specific blocking antibodies. Our data strongly suggest that DST induces not only the differentiation of suppressor cells and production of anti-idiotypic antibodies, but also the appearance of non-specific, non-cytotoxic antibodies, which may participate in the cell-mediated immune suppression of the alloimmune reactivity.

Original languageEnglish (US)
Pages (from-to)127-130
Number of pages4
JournalImmunology Letters
Volume26
Issue number2
DOIs
Publication statusPublished - Nov 1990
Externally publishedYes

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Keywords

  • Blocking antibody
  • Blood transfusion
  • Immunosuppression
  • Suppressor cell

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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