Novel bioactive root canal sealer with antibiofilm and remineralization properties

Bashayer H. Baras, Suping Wang, Mary Anne S. Melo, Franklin Chi Meng Tay, Ashraf F. Fouad, Dwayne D. Arola, Michael D. Weir, Hockin H.K. Xu

Research output: Contribution to journalArticle

Abstract

Objectives: (1) To develop a novel bioactive root canal sealer with antibiofilm and remineralization properties using dimethylaminohexadecyl methacrylate (DMAHDM) and nanoparticles of amorphous calcium phosphate (NACP); (2) investigate the effects on E. faecalis biofilm inhibition, sealer flow and sealing ability, compared with an epoxy-resin-based sealer AH Plus; and (3) investigate the calcium (Ca) and phosphate (P) ion release from the sealers. Methods: A series of dual-cure endodontic sealers were formulated with DMAHDM and NACP at 5% and 20% by mass, respectively. Flow properties and sealing ability of the sealers were measured. Colony-forming units (CFU), live/dead assay, and polysaccharide production of biofilms on sealers were determined. Ca and P ion releases from the sealers were measured. Results: The new sealer containing 20% NACP and 5% DMAHDM yielded a paste flow of (28.99 ± 0.69) mm, within the range of ISO recommendations. The sealing properties of the sealer with 5% DMAHDM and 20% NACP were similar to a commercial control (p > 0.05). The sealer with DMAHDM decreased E. faecalis biofilm CFU by more than 4 orders of magnitude, compared to AH plus and experimental controls. The sealer with 20% NACP and 5% DMAHDM had relatively high levels of Ca and P ion release necessary for remineralization. Conclusions: A new bioactive endodontic sealer was developed with strong antibiofilm activity against E. faecalis biofilms and high levels of Ca and P ion release for remineralization, without compromising the paste flow and sealing properties. Clinical significance: The bioactive antibacterial and remineralizing root canal sealer is promising to inhibit E. faecalis biofilms to prevent endodontic treatment failure and secondary endodontic infections, while releasing high levels of Ca and P ions that could remineralize and strengthen the tooth structures and potentially prevent future root fractures and teeth extractions.

Original languageEnglish (US)
Pages (from-to)67-76
Number of pages10
JournalJournal of Dentistry
Volume83
DOIs
StatePublished - Apr 1 2019

Fingerprint

Methacrylates
Dental Pulp Cavity
Biofilms
Nanoparticles
Endodontics
Ions
Calcium
Ointments
Stem Cells
Epoxy Resins
Tooth Extraction
Treatment Failure
Coinfection
Polysaccharides
canals sealer
Tooth
amorphous calcium phosphate

Keywords

  • Antibacterial
  • Calcium phosphate nanoparticles
  • Endodontic biofilms
  • Microleakage
  • Root canal sealer
  • Sealing ability

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Baras, B. H., Wang, S., Melo, M. A. S., Tay, F. C. M., Fouad, A. F., Arola, D. D., ... Xu, H. H. K. (2019). Novel bioactive root canal sealer with antibiofilm and remineralization properties. Journal of Dentistry, 83, 67-76. https://doi.org/10.1016/j.jdent.2019.02.006

Novel bioactive root canal sealer with antibiofilm and remineralization properties. / Baras, Bashayer H.; Wang, Suping; Melo, Mary Anne S.; Tay, Franklin Chi Meng; Fouad, Ashraf F.; Arola, Dwayne D.; Weir, Michael D.; Xu, Hockin H.K.

In: Journal of Dentistry, Vol. 83, 01.04.2019, p. 67-76.

Research output: Contribution to journalArticle

Baras, BH, Wang, S, Melo, MAS, Tay, FCM, Fouad, AF, Arola, DD, Weir, MD & Xu, HHK 2019, 'Novel bioactive root canal sealer with antibiofilm and remineralization properties', Journal of Dentistry, vol. 83, pp. 67-76. https://doi.org/10.1016/j.jdent.2019.02.006
Baras, Bashayer H. ; Wang, Suping ; Melo, Mary Anne S. ; Tay, Franklin Chi Meng ; Fouad, Ashraf F. ; Arola, Dwayne D. ; Weir, Michael D. ; Xu, Hockin H.K. / Novel bioactive root canal sealer with antibiofilm and remineralization properties. In: Journal of Dentistry. 2019 ; Vol. 83. pp. 67-76.
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abstract = "Objectives: (1) To develop a novel bioactive root canal sealer with antibiofilm and remineralization properties using dimethylaminohexadecyl methacrylate (DMAHDM) and nanoparticles of amorphous calcium phosphate (NACP); (2) investigate the effects on E. faecalis biofilm inhibition, sealer flow and sealing ability, compared with an epoxy-resin-based sealer AH Plus; and (3) investigate the calcium (Ca) and phosphate (P) ion release from the sealers. Methods: A series of dual-cure endodontic sealers were formulated with DMAHDM and NACP at 5{\%} and 20{\%} by mass, respectively. Flow properties and sealing ability of the sealers were measured. Colony-forming units (CFU), live/dead assay, and polysaccharide production of biofilms on sealers were determined. Ca and P ion releases from the sealers were measured. Results: The new sealer containing 20{\%} NACP and 5{\%} DMAHDM yielded a paste flow of (28.99 ± 0.69) mm, within the range of ISO recommendations. The sealing properties of the sealer with 5{\%} DMAHDM and 20{\%} NACP were similar to a commercial control (p > 0.05). The sealer with DMAHDM decreased E. faecalis biofilm CFU by more than 4 orders of magnitude, compared to AH plus and experimental controls. The sealer with 20{\%} NACP and 5{\%} DMAHDM had relatively high levels of Ca and P ion release necessary for remineralization. Conclusions: A new bioactive endodontic sealer was developed with strong antibiofilm activity against E. faecalis biofilms and high levels of Ca and P ion release for remineralization, without compromising the paste flow and sealing properties. Clinical significance: The bioactive antibacterial and remineralizing root canal sealer is promising to inhibit E. faecalis biofilms to prevent endodontic treatment failure and secondary endodontic infections, while releasing high levels of Ca and P ions that could remineralize and strengthen the tooth structures and potentially prevent future root fractures and teeth extractions.",
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