Novel expression of CD11b in epithelial ovarian cancer: Potential therapeutic target

Ghassan M. Saed, Nicole M. Fletcher, Michael P. Diamond, Robert T. Morris, Nardhy Gomez-Lopez, Ira Memaj

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Objective: The objective of this study was to determine the expression, and effect of targeting CD11b with a monoclonal antibody in ovarian cancer cells. Methods: CD11b expression was determined in epithelial ovarian cancer (EOC) cell lines and tissues by immunofluorescence and flow cytometry. Cytotoxicity of the CD11b antibody and synergism with chemothearapeutic drugs were determined by the MTT Cell Proliferation Assay in human macrophages, normal ovarian epithelial cells, and in both sensitive and chemoresistant EOC cell lines. Cell migration was assessed with a scratch assay and in vivo effects of the CD11b antibody was assessed with a nude mouse ovarian cancer xenograft model. Data was analyzed with either t-tests or one-way ANOVA. Results: CD11b was unexpectedly expressed in several EOC lines and tissues, but not normal tissues. Targeting CD11b with its monoclonal antibody resulted in intriguing cytotoxic effects in sensitive and chemoresistant EOC lines, while surprisingly not affecting normal cells. More importantly, the cytotoxicity of the CD11b antibody when combined with chemotherapeutic drugs (cisplatin or docetaxel) was significantly synergistic, in both sensitive and chemoresistant EOC cells. The anti-tumorigenic effect of the CD11b antibody was confirmed in an ovarian cancer nude mouse xenograft model. Conclusion: Here we identify CD11b as a novel target, which selectively induces cytotoxicity in ovarian cancer cells.

Original languageEnglish (US)
Pages (from-to)567-575
Number of pages9
JournalGynecologic Oncology
Volume148
Issue number3
DOIs
StatePublished - Mar 2018

Keywords

  • Apoptosis
  • CD11b
  • CD11b antibody
  • Cytotoxicity
  • Myeloperoxidase
  • Survival mechanism

ASJC Scopus subject areas

  • Oncology
  • Obstetrics and Gynecology

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