Novel HPV types present in oral papillomatous lesions from patients with HIV infection

Christiane Völter, Yukai He, Hajo Delius, Arup Roy-Burman, John S. Greenspan, Deborah Greenspan, Ethel Michele De Villiers

Research output: Contribution to journalArticle

82 Citations (Scopus)

Abstract

Patients infected with the human immunodeficiency virus (HIV) often develop multiple papillomatous lesions of the oral cavity. In the present study, a total of 67 biopsies from benign oral lesions were analyzed for the presence of human papillomavirus (HPV) DNA using Southern-blot hybridization in combination with a polymerase chain reaction designed to detect all known HPV types, as well as unidentified types. These samples, collected at random from a high-risk population, were subsequently divided into 57 biopsies originating from patients with confirmed HIV infection and 10 biopsies from patients with unknown HIV status. Each sample was amplified with 7 different combinations of degenerate primers. An amplified products were sequenced. HPV DNA sequences were detected in 67% (45/67) of the samples. HPV 7 (19%) and HPV 32 (28%) were the predominant HPV types. HPV 32 was present in 2/4 fibromas tested. Two new HPV types, HPV 72 and HPV 73, were identified in oral warts with atypia. The complete genomes of these viruses were cloned and sequenced. Other HPV types detected were HPV 2a, HPV 6b, HPV 13, HPV 16, HPV 18, HPV 55, HPV 59 and HPV 69.

Original languageEnglish (US)
Pages (from-to)453-456
Number of pages4
JournalInternational Journal of Cancer
Volume66
Issue number4
DOIs
StatePublished - May 16 1996

Fingerprint

Virus Diseases
HIV
Biopsy
Human papillomavirus 18
Warts
Fibroma
Human papillomavirus 16
Southern Blotting

ASJC Scopus subject areas

  • Medicine(all)
  • Oncology
  • Cancer Research

Cite this

Novel HPV types present in oral papillomatous lesions from patients with HIV infection. / Völter, Christiane; He, Yukai; Delius, Hajo; Roy-Burman, Arup; Greenspan, John S.; Greenspan, Deborah; De Villiers, Ethel Michele.

In: International Journal of Cancer, Vol. 66, No. 4, 16.05.1996, p. 453-456.

Research output: Contribution to journalArticle

Völter, Christiane ; He, Yukai ; Delius, Hajo ; Roy-Burman, Arup ; Greenspan, John S. ; Greenspan, Deborah ; De Villiers, Ethel Michele. / Novel HPV types present in oral papillomatous lesions from patients with HIV infection. In: International Journal of Cancer. 1996 ; Vol. 66, No. 4. pp. 453-456.
@article{2e16228211254cf6b87b9e1cb133b89d,
title = "Novel HPV types present in oral papillomatous lesions from patients with HIV infection",
abstract = "Patients infected with the human immunodeficiency virus (HIV) often develop multiple papillomatous lesions of the oral cavity. In the present study, a total of 67 biopsies from benign oral lesions were analyzed for the presence of human papillomavirus (HPV) DNA using Southern-blot hybridization in combination with a polymerase chain reaction designed to detect all known HPV types, as well as unidentified types. These samples, collected at random from a high-risk population, were subsequently divided into 57 biopsies originating from patients with confirmed HIV infection and 10 biopsies from patients with unknown HIV status. Each sample was amplified with 7 different combinations of degenerate primers. An amplified products were sequenced. HPV DNA sequences were detected in 67{\%} (45/67) of the samples. HPV 7 (19{\%}) and HPV 32 (28{\%}) were the predominant HPV types. HPV 32 was present in 2/4 fibromas tested. Two new HPV types, HPV 72 and HPV 73, were identified in oral warts with atypia. The complete genomes of these viruses were cloned and sequenced. Other HPV types detected were HPV 2a, HPV 6b, HPV 13, HPV 16, HPV 18, HPV 55, HPV 59 and HPV 69.",
author = "Christiane V{\"o}lter and Yukai He and Hajo Delius and Arup Roy-Burman and Greenspan, {John S.} and Deborah Greenspan and {De Villiers}, {Ethel Michele}",
year = "1996",
month = "5",
day = "16",
doi = "10.1002/(SICI)1097-0215(19960516)66:4<453::AID-IJC7>3.0.CO;2-V",
language = "English (US)",
volume = "66",
pages = "453--456",
journal = "International Journal of Cancer",
issn = "0020-7136",
publisher = "Wiley-Liss Inc.",
number = "4",

}

TY - JOUR

T1 - Novel HPV types present in oral papillomatous lesions from patients with HIV infection

AU - Völter, Christiane

AU - He, Yukai

AU - Delius, Hajo

AU - Roy-Burman, Arup

AU - Greenspan, John S.

AU - Greenspan, Deborah

AU - De Villiers, Ethel Michele

PY - 1996/5/16

Y1 - 1996/5/16

N2 - Patients infected with the human immunodeficiency virus (HIV) often develop multiple papillomatous lesions of the oral cavity. In the present study, a total of 67 biopsies from benign oral lesions were analyzed for the presence of human papillomavirus (HPV) DNA using Southern-blot hybridization in combination with a polymerase chain reaction designed to detect all known HPV types, as well as unidentified types. These samples, collected at random from a high-risk population, were subsequently divided into 57 biopsies originating from patients with confirmed HIV infection and 10 biopsies from patients with unknown HIV status. Each sample was amplified with 7 different combinations of degenerate primers. An amplified products were sequenced. HPV DNA sequences were detected in 67% (45/67) of the samples. HPV 7 (19%) and HPV 32 (28%) were the predominant HPV types. HPV 32 was present in 2/4 fibromas tested. Two new HPV types, HPV 72 and HPV 73, were identified in oral warts with atypia. The complete genomes of these viruses were cloned and sequenced. Other HPV types detected were HPV 2a, HPV 6b, HPV 13, HPV 16, HPV 18, HPV 55, HPV 59 and HPV 69.

AB - Patients infected with the human immunodeficiency virus (HIV) often develop multiple papillomatous lesions of the oral cavity. In the present study, a total of 67 biopsies from benign oral lesions were analyzed for the presence of human papillomavirus (HPV) DNA using Southern-blot hybridization in combination with a polymerase chain reaction designed to detect all known HPV types, as well as unidentified types. These samples, collected at random from a high-risk population, were subsequently divided into 57 biopsies originating from patients with confirmed HIV infection and 10 biopsies from patients with unknown HIV status. Each sample was amplified with 7 different combinations of degenerate primers. An amplified products were sequenced. HPV DNA sequences were detected in 67% (45/67) of the samples. HPV 7 (19%) and HPV 32 (28%) were the predominant HPV types. HPV 32 was present in 2/4 fibromas tested. Two new HPV types, HPV 72 and HPV 73, were identified in oral warts with atypia. The complete genomes of these viruses were cloned and sequenced. Other HPV types detected were HPV 2a, HPV 6b, HPV 13, HPV 16, HPV 18, HPV 55, HPV 59 and HPV 69.

UR - http://www.scopus.com/inward/record.url?scp=0029950979&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029950979&partnerID=8YFLogxK

U2 - 10.1002/(SICI)1097-0215(19960516)66:4<453::AID-IJC7>3.0.CO;2-V

DO - 10.1002/(SICI)1097-0215(19960516)66:4<453::AID-IJC7>3.0.CO;2-V

M3 - Article

C2 - 8635859

AN - SCOPUS:0029950979

VL - 66

SP - 453

EP - 456

JO - International Journal of Cancer

JF - International Journal of Cancer

SN - 0020-7136

IS - 4

ER -