Abstract
The Caulobacter crescentus flaFG region encodes trans-acting, regulatory factors that modulates flagellin synthesis during flagellum biogenesis. In this study, sequence analysis and experiments utilizing a promoterless cat gene demonstrated that the flaF and flbT genes have overlapping transcripts with the same orientation. In addition, the 5′ ends of the flgL and flbA genes were located. A sequence resembling an Rho-factor-independent terminator was found in the 3′ region of the flaF gene. This region was uniquely A + T-rich and the encoded mRNA contained an inverted repeat sequence which could form a stable stem-loop strucuture followed by nine U-residues. The codon usage of C. crescentus genes was examined and indicated a preference for specific codons from each of the synonymous codon groups. Furthermore, comparison to the codon usage of other organisms with G + C-rich genomes indicated a strong preference for the same codons prefered by C. crescentus.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 17-25 |
| Number of pages | 9 |
| Journal | Gene |
| Volume | 93 |
| Issue number | 1 |
| DOIs | |
| State | Published - Sep 1 1990 |
| Externally published | Yes |
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Keywords
- Recombinant DNA
- cat gene
- cloning
- flagellar genes
- motility
- overlapping genes
ASJC Scopus subject areas
- Genetics
Cite this
Nucleotide sequence of the Caulobacter crescents flaF and flbt genes and analysis of codon usage in organisms with G+C-rich genomes. / Schoenlein, Patricia V; Gallman, Lilly S.; Winkler, Malcolm E.; Ely, Bert.
In: Gene, Vol. 93, No. 1, 01.09.1990, p. 17-25.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Nucleotide sequence of the Caulobacter crescents flaF and flbt genes and analysis of codon usage in organisms with G+C-rich genomes
AU - Schoenlein, Patricia V
AU - Gallman, Lilly S.
AU - Winkler, Malcolm E.
AU - Ely, Bert
PY - 1990/9/1
Y1 - 1990/9/1
N2 - The Caulobacter crescentus flaFG region encodes trans-acting, regulatory factors that modulates flagellin synthesis during flagellum biogenesis. In this study, sequence analysis and experiments utilizing a promoterless cat gene demonstrated that the flaF and flbT genes have overlapping transcripts with the same orientation. In addition, the 5′ ends of the flgL and flbA genes were located. A sequence resembling an Rho-factor-independent terminator was found in the 3′ region of the flaF gene. This region was uniquely A + T-rich and the encoded mRNA contained an inverted repeat sequence which could form a stable stem-loop strucuture followed by nine U-residues. The codon usage of C. crescentus genes was examined and indicated a preference for specific codons from each of the synonymous codon groups. Furthermore, comparison to the codon usage of other organisms with G + C-rich genomes indicated a strong preference for the same codons prefered by C. crescentus.
AB - The Caulobacter crescentus flaFG region encodes trans-acting, regulatory factors that modulates flagellin synthesis during flagellum biogenesis. In this study, sequence analysis and experiments utilizing a promoterless cat gene demonstrated that the flaF and flbT genes have overlapping transcripts with the same orientation. In addition, the 5′ ends of the flgL and flbA genes were located. A sequence resembling an Rho-factor-independent terminator was found in the 3′ region of the flaF gene. This region was uniquely A + T-rich and the encoded mRNA contained an inverted repeat sequence which could form a stable stem-loop strucuture followed by nine U-residues. The codon usage of C. crescentus genes was examined and indicated a preference for specific codons from each of the synonymous codon groups. Furthermore, comparison to the codon usage of other organisms with G + C-rich genomes indicated a strong preference for the same codons prefered by C. crescentus.
KW - Recombinant DNA
KW - cat gene
KW - cloning
KW - flagellar genes
KW - motility
KW - overlapping genes
UR - http://www.scopus.com/inward/record.url?scp=0025107760&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0025107760&partnerID=8YFLogxK
U2 - 10.1016/0378-1119(90)90130-J
DO - 10.1016/0378-1119(90)90130-J
M3 - Article
C2 - 1699845
AN - SCOPUS:0025107760
VL - 93
SP - 17
EP - 25
JO - Gene
JF - Gene
SN - 0378-1119
IS - 1
ER -