Nucleotide sequence of the Caulobacter crescents flaF and flbt genes and analysis of codon usage in organisms with G+C-rich genomes

Patricia V Schoenlein, Lilly S. Gallman, Malcolm E. Winkler, Bert Ely

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The Caulobacter crescentus flaFG region encodes trans-acting, regulatory factors that modulates flagellin synthesis during flagellum biogenesis. In this study, sequence analysis and experiments utilizing a promoterless cat gene demonstrated that the flaF and flbT genes have overlapping transcripts with the same orientation. In addition, the 5′ ends of the flgL and flbA genes were located. A sequence resembling an Rho-factor-independent terminator was found in the 3′ region of the flaF gene. This region was uniquely A + T-rich and the encoded mRNA contained an inverted repeat sequence which could form a stable stem-loop strucuture followed by nine U-residues. The codon usage of C. crescentus genes was examined and indicated a preference for specific codons from each of the synonymous codon groups. Furthermore, comparison to the codon usage of other organisms with G + C-rich genomes indicated a strong preference for the same codons prefered by C. crescentus.

Original languageEnglish (US)
Pages (from-to)17-25
Number of pages9
JournalGene
Volume93
Issue number1
DOIs
StatePublished - Sep 1 1990
Externally publishedYes

Fingerprint

Caulobacter
Codon
Caulobacter crescentus
Genome
Genes
Rho Factor
Overlapping Genes
Inverted Repeat Sequences
Flagellin
Trans-Activators
Flagella
Sequence Analysis
Cats
Messenger RNA

Keywords

  • Recombinant DNA
  • cat gene
  • cloning
  • flagellar genes
  • motility
  • overlapping genes

ASJC Scopus subject areas

  • Genetics

Cite this

Nucleotide sequence of the Caulobacter crescents flaF and flbt genes and analysis of codon usage in organisms with G+C-rich genomes. / Schoenlein, Patricia V; Gallman, Lilly S.; Winkler, Malcolm E.; Ely, Bert.

In: Gene, Vol. 93, No. 1, 01.09.1990, p. 17-25.

Research output: Contribution to journalArticle

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AB - The Caulobacter crescentus flaFG region encodes trans-acting, regulatory factors that modulates flagellin synthesis during flagellum biogenesis. In this study, sequence analysis and experiments utilizing a promoterless cat gene demonstrated that the flaF and flbT genes have overlapping transcripts with the same orientation. In addition, the 5′ ends of the flgL and flbA genes were located. A sequence resembling an Rho-factor-independent terminator was found in the 3′ region of the flaF gene. This region was uniquely A + T-rich and the encoded mRNA contained an inverted repeat sequence which could form a stable stem-loop strucuture followed by nine U-residues. The codon usage of C. crescentus genes was examined and indicated a preference for specific codons from each of the synonymous codon groups. Furthermore, comparison to the codon usage of other organisms with G + C-rich genomes indicated a strong preference for the same codons prefered by C. crescentus.

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