O-acetylated gangliosides in bovine buttermilk. Characterization of 7-O- acetyl, 9-O-acetyl, and 7,9-di-O-acetyl G(D3)

S. Ren, J. N. Scarsdale, T. Ariga, Y. Zhang, R. A. Klein, R. Hartmann, Y. Kushi, H. Egge, R. K. Yu

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54 Scopus citations

Abstract

Three O-acetylated gangliosides, G1, G2, and G3, were purified from bovine buttermilk by using chloroform/methanol extraction, Folch partitioning, chromatography on DEAE-Sephadex A-25, and Iatrobeads columns. The final yields of gangliosides G1, G2, and G3 were 2 mg, 37 mg, and 40 mg per 1.7 kg of the buttermilk powder, respectively. On the basis of immunostaining on high performance thin layer chromatography with specific monoclonal antibodies, mild alkaline treatment, gas-liquid chromatographic analysis, fast atom bombardment mass spectrometry, and proton nuclear magnetic resonance studies, G1 and G2 are characterized as O-acetylated G(D3) and G3 as O-acetylated G(T3). The major fatty acids of these gangliosides were C18:0, C22:0, C23:0, and C24:0, and the long chain base was C18-sphingosine.

Original languageEnglish (US)
Pages (from-to)12632-12638
Number of pages7
JournalJournal of Biological Chemistry
Volume267
Issue number18
StatePublished - Jul 15 1992
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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    Ren, S., Scarsdale, J. N., Ariga, T., Zhang, Y., Klein, R. A., Hartmann, R., Kushi, Y., Egge, H., & Yu, R. K. (1992). O-acetylated gangliosides in bovine buttermilk. Characterization of 7-O- acetyl, 9-O-acetyl, and 7,9-di-O-acetyl G(D3). Journal of Biological Chemistry, 267(18), 12632-12638.