TY - JOUR
T1 - O-acetylated gangliosides in bovine buttermilk
T2 - Characterization of 7-O-acetyl, 9-O-acetyl, and 7,9-DI-O-acetyl GD3
AU - Ren, Shunlin
AU - Scarsdale, J. Neel
AU - Ariga, Toshio
AU - Zhang, Yuejin
AU - Klein, Roger A.
AU - Hartmann, Rudolf
AU - Kushi, Yasunori
AU - Egge, Heinz
AU - Yu, Robert K.
N1 - Copyright:
Copyright 2007 Elsevier B.V., All rights reserved.
PY - 1992/6/25
Y1 - 1992/6/25
N2 - Three O-acetylated gangliosides, G1, G2, and G3, were purified from bovine buttermilk by using chloroform/methanol extraction, Folch partitioning, chromatography on DEAE-Sephadex A-25, and Iatrobeads columns. The final yields of gangliosides G1, G2, and G3 were 2 mg, 37 mg, and 40 mg per 1.7 kg of the buttermilk powder, respectively. On the basis of immunostaining on high performance thin layer chromatography with specific monoclonal antibodies, mild alkaline treatment, gas-liquid Chromatographie analysis, fast atom bombardment mass spectrometry, and proton nuclear magnetic resonance studies, G1 and G2 are characterized as O-acetylated GD3 and G3 as O-acetylated GT3, and the structures of these gangliosides are as follows: equation presented The major fatty acids of these gangliosides were C 18:0, C22:0, C23:0, and C24:0, and the long chain base was C18-sphingosine.
AB - Three O-acetylated gangliosides, G1, G2, and G3, were purified from bovine buttermilk by using chloroform/methanol extraction, Folch partitioning, chromatography on DEAE-Sephadex A-25, and Iatrobeads columns. The final yields of gangliosides G1, G2, and G3 were 2 mg, 37 mg, and 40 mg per 1.7 kg of the buttermilk powder, respectively. On the basis of immunostaining on high performance thin layer chromatography with specific monoclonal antibodies, mild alkaline treatment, gas-liquid Chromatographie analysis, fast atom bombardment mass spectrometry, and proton nuclear magnetic resonance studies, G1 and G2 are characterized as O-acetylated GD3 and G3 as O-acetylated GT3, and the structures of these gangliosides are as follows: equation presented The major fatty acids of these gangliosides were C 18:0, C22:0, C23:0, and C24:0, and the long chain base was C18-sphingosine.
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M3 - Article
C2 - 1618769
AN - SCOPUS:0026734155
SN - 0021-9258
VL - 267
SP - 12632
EP - 12638
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 18
ER -