O-acetylated gangliosides in bovine buttermilk. Characterization of 7-O- acetyl, 9-O-acetyl, and 7,9-di-O-acetyl G(D3)

S. Ren, J. N. Scarsdale, T. Ariga, Y. Zhang, R. A. Klein, R. Hartmann, Y. Kushi, H. Egge, Robert K Yu

Research output: Contribution to journalArticle

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Abstract

Three O-acetylated gangliosides, G1, G2, and G3, were purified from bovine buttermilk by using chloroform/methanol extraction, Folch partitioning, chromatography on DEAE-Sephadex A-25, and Iatrobeads columns. The final yields of gangliosides G1, G2, and G3 were 2 mg, 37 mg, and 40 mg per 1.7 kg of the buttermilk powder, respectively. On the basis of immunostaining on high performance thin layer chromatography with specific monoclonal antibodies, mild alkaline treatment, gas-liquid chromatographic analysis, fast atom bombardment mass spectrometry, and proton nuclear magnetic resonance studies, G1 and G2 are characterized as O-acetylated G(D3) and G3 as O-acetylated G(T3). The major fatty acids of these gangliosides were C18:0, C22:0, C23:0, and C24:0, and the long chain base was C18-sphingosine.

Original languageEnglish (US)
Pages (from-to)12632-12638
Number of pages7
JournalJournal of Biological Chemistry
Volume267
Issue number18
StatePublished - Jul 15 1992
Externally publishedYes

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Gangliosides
Nuclear magnetic resonance
Fast Atom Bombardment Mass Spectrometry
Thin layer chromatography
Sphingosine
Liquid chromatography
Thin Layer Chromatography
Chloroform
Chromatography
Gas Chromatography
Powders
Mass spectrometry
Methanol
Protons
Magnetic Resonance Spectroscopy
Fatty Acids
Gases
Monoclonal Antibodies
Atoms
Buttermilk

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Ren, S., Scarsdale, J. N., Ariga, T., Zhang, Y., Klein, R. A., Hartmann, R., ... Yu, R. K. (1992). O-acetylated gangliosides in bovine buttermilk. Characterization of 7-O- acetyl, 9-O-acetyl, and 7,9-di-O-acetyl G(D3). Journal of Biological Chemistry, 267(18), 12632-12638.

O-acetylated gangliosides in bovine buttermilk. Characterization of 7-O- acetyl, 9-O-acetyl, and 7,9-di-O-acetyl G(D3). / Ren, S.; Scarsdale, J. N.; Ariga, T.; Zhang, Y.; Klein, R. A.; Hartmann, R.; Kushi, Y.; Egge, H.; Yu, Robert K.

In: Journal of Biological Chemistry, Vol. 267, No. 18, 15.07.1992, p. 12632-12638.

Research output: Contribution to journalArticle

Ren, S, Scarsdale, JN, Ariga, T, Zhang, Y, Klein, RA, Hartmann, R, Kushi, Y, Egge, H & Yu, RK 1992, 'O-acetylated gangliosides in bovine buttermilk. Characterization of 7-O- acetyl, 9-O-acetyl, and 7,9-di-O-acetyl G(D3)', Journal of Biological Chemistry, vol. 267, no. 18, pp. 12632-12638.
Ren S, Scarsdale JN, Ariga T, Zhang Y, Klein RA, Hartmann R et al. O-acetylated gangliosides in bovine buttermilk. Characterization of 7-O- acetyl, 9-O-acetyl, and 7,9-di-O-acetyl G(D3). Journal of Biological Chemistry. 1992 Jul 15;267(18):12632-12638.
Ren, S. ; Scarsdale, J. N. ; Ariga, T. ; Zhang, Y. ; Klein, R. A. ; Hartmann, R. ; Kushi, Y. ; Egge, H. ; Yu, Robert K. / O-acetylated gangliosides in bovine buttermilk. Characterization of 7-O- acetyl, 9-O-acetyl, and 7,9-di-O-acetyl G(D3). In: Journal of Biological Chemistry. 1992 ; Vol. 267, No. 18. pp. 12632-12638.
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AU - Scarsdale, J. N.

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AU - Zhang, Y.

AU - Klein, R. A.

AU - Hartmann, R.

AU - Kushi, Y.

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