Orphanin FQ inhibits GnRH secretion from rat hypothalamic fragments but not GTI-7 neurons

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Orphanin FQ is a novel opioid family member, which is densely localized in the hypothalamus, a region of the brain important for the control of reproduction. This study tested the hypothesis that orphanin FQ might regulate the secretion of gonadotropin-releasing hormone, the key central regulator of the ovulatory cycle. To test this hypothesis, we used rat hypothalamic fragments and immortalized gonadotropin-releasing hormone neurons (GTI-7) in vitro and examined whether orphanin FQ would inhibit forskolin-induced gonadotropin-releasing hormone release. The studies revealed that orphanin FQ potently and dose-dependently inhibits forskolin-induced gonadotropin-releasing hormone release from rat hypothalamic fragments. In contrast, orphanin FQ had no effect on gonadotropin-releasing hormone release from GTI-7 neurons. Reverse transcriptase-polymerase chain reaction analysis further revealed that the orphanin FQ receptor, ORL-1 is expressed in hypothalamic fragments, but not in GTI-7 neurons. Together, these findings are the first to suggest a role for orphanin FQ in the regulation of gonadotropin releasing hormone secretion.

Original languageEnglish (US)
Pages (from-to)1247-1249
Number of pages3
JournalNeuroReport
Volume13
Issue number10
DOIs
StatePublished - Jul 19 2002

Fingerprint

Gonadotropin-Releasing Hormone
Neurons
Colforsin
Reverse Transcriptase Polymerase Chain Reaction
Opioid Analgesics
Hypothalamus
Reproduction
nociceptin
Brain

Keywords

  • GnRH
  • GTI-7 cells
  • Hypothalamus
  • ORL-I
  • Orphanin FQ

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Orphanin FQ inhibits GnRH secretion from rat hypothalamic fragments but not GTI-7 neurons. / Dhandapani, Krishnan Michael; Brann, Darrell W.

In: NeuroReport, Vol. 13, No. 10, 19.07.2002, p. 1247-1249.

Research output: Contribution to journalArticle

@article{a8fb4d631a584c918b62ff0df855b2e9,
title = "Orphanin FQ inhibits GnRH secretion from rat hypothalamic fragments but not GTI-7 neurons",
abstract = "Orphanin FQ is a novel opioid family member, which is densely localized in the hypothalamus, a region of the brain important for the control of reproduction. This study tested the hypothesis that orphanin FQ might regulate the secretion of gonadotropin-releasing hormone, the key central regulator of the ovulatory cycle. To test this hypothesis, we used rat hypothalamic fragments and immortalized gonadotropin-releasing hormone neurons (GTI-7) in vitro and examined whether orphanin FQ would inhibit forskolin-induced gonadotropin-releasing hormone release. The studies revealed that orphanin FQ potently and dose-dependently inhibits forskolin-induced gonadotropin-releasing hormone release from rat hypothalamic fragments. In contrast, orphanin FQ had no effect on gonadotropin-releasing hormone release from GTI-7 neurons. Reverse transcriptase-polymerase chain reaction analysis further revealed that the orphanin FQ receptor, ORL-1 is expressed in hypothalamic fragments, but not in GTI-7 neurons. Together, these findings are the first to suggest a role for orphanin FQ in the regulation of gonadotropin releasing hormone secretion.",
keywords = "GnRH, GTI-7 cells, Hypothalamus, ORL-I, Orphanin FQ",
author = "Dhandapani, {Krishnan Michael} and Brann, {Darrell W}",
year = "2002",
month = "7",
day = "19",
doi = "10.1097/00001756-200207190-00005",
language = "English (US)",
volume = "13",
pages = "1247--1249",
journal = "NeuroReport",
issn = "0959-4965",
publisher = "Lippincott Williams and Wilkins",
number = "10",

}

TY - JOUR

T1 - Orphanin FQ inhibits GnRH secretion from rat hypothalamic fragments but not GTI-7 neurons

AU - Dhandapani, Krishnan Michael

AU - Brann, Darrell W

PY - 2002/7/19

Y1 - 2002/7/19

N2 - Orphanin FQ is a novel opioid family member, which is densely localized in the hypothalamus, a region of the brain important for the control of reproduction. This study tested the hypothesis that orphanin FQ might regulate the secretion of gonadotropin-releasing hormone, the key central regulator of the ovulatory cycle. To test this hypothesis, we used rat hypothalamic fragments and immortalized gonadotropin-releasing hormone neurons (GTI-7) in vitro and examined whether orphanin FQ would inhibit forskolin-induced gonadotropin-releasing hormone release. The studies revealed that orphanin FQ potently and dose-dependently inhibits forskolin-induced gonadotropin-releasing hormone release from rat hypothalamic fragments. In contrast, orphanin FQ had no effect on gonadotropin-releasing hormone release from GTI-7 neurons. Reverse transcriptase-polymerase chain reaction analysis further revealed that the orphanin FQ receptor, ORL-1 is expressed in hypothalamic fragments, but not in GTI-7 neurons. Together, these findings are the first to suggest a role for orphanin FQ in the regulation of gonadotropin releasing hormone secretion.

AB - Orphanin FQ is a novel opioid family member, which is densely localized in the hypothalamus, a region of the brain important for the control of reproduction. This study tested the hypothesis that orphanin FQ might regulate the secretion of gonadotropin-releasing hormone, the key central regulator of the ovulatory cycle. To test this hypothesis, we used rat hypothalamic fragments and immortalized gonadotropin-releasing hormone neurons (GTI-7) in vitro and examined whether orphanin FQ would inhibit forskolin-induced gonadotropin-releasing hormone release. The studies revealed that orphanin FQ potently and dose-dependently inhibits forskolin-induced gonadotropin-releasing hormone release from rat hypothalamic fragments. In contrast, orphanin FQ had no effect on gonadotropin-releasing hormone release from GTI-7 neurons. Reverse transcriptase-polymerase chain reaction analysis further revealed that the orphanin FQ receptor, ORL-1 is expressed in hypothalamic fragments, but not in GTI-7 neurons. Together, these findings are the first to suggest a role for orphanin FQ in the regulation of gonadotropin releasing hormone secretion.

KW - GnRH

KW - GTI-7 cells

KW - Hypothalamus

KW - ORL-I

KW - Orphanin FQ

UR - http://www.scopus.com/inward/record.url?scp=0037135218&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037135218&partnerID=8YFLogxK

U2 - 10.1097/00001756-200207190-00005

DO - 10.1097/00001756-200207190-00005

M3 - Article

C2 - 12151779

AN - SCOPUS:0037135218

VL - 13

SP - 1247

EP - 1249

JO - NeuroReport

JF - NeuroReport

SN - 0959-4965

IS - 10

ER -