Over-expression of HSP-70 protects astrocytes from combined oxygen-glucose deprivation

Marios C. Papadopoulos, Xiao Yun Sun, Jianmin Cao, Nahid F Mivechi, Rona G. Giffard

Research output: Contribution to journalArticle

74 Scopus citations

Abstract

Pretreatment by a sublethal insult is associated with induction of stress proteins and with protection from subsequent injury. Heat pretreatment protects the brain from subsequent ischemia, and is shown here to protect primary astrocyte cultures from subsequent oxygen-glucose deprivation. To determine whether the expression of a single stress protein, HSP-70, could account for much of this protection, we expressed HSP-70 or β-galactosidase in astrocytes using retroviral vectors. Only 12% of astrocytes expressing HSP-70 died after 7 hours of oxygen-glucose deprivation compared to 65% of astrocytes expressing β-galactosidase and 82% of normal astrocytes. Our data provide direct evidence that selective expression of HSP-70 enhances the survival of astrocytes challenged with heat or oxygen-glucose deprivation.

Original languageEnglish (US)
Pages (from-to)429-432
Number of pages4
JournalNeuroReport
Volume7
Issue number2
DOIs
Publication statusPublished - Jan 1 1996
Externally publishedYes

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Keywords

  • astrocytes
  • heat shock proteins
  • hyperthermia
  • ischemia
  • mouse
  • retrovirus

ASJC Scopus subject areas

  • Neuroscience(all)

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