Oxygen derived radicals related injury in the heart during calcium paradox

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Abstract

The effects of oxygen-derived radical scavengers (ODRS) on the heart was investigated during the calcium paradox. Perfusion with Ca2 +-free medium caused cell separation at the intercalated discs and changes in the endothelial cells. Upon Ca2+ reintroduction, a massive cell damage occurred. The cytosolic enzyme, creatine phosphokinase (CPK), was released in large amounts (p< 0.001). The tissue adenosine triphosphate (ATP) was reduced to 3.7 μmol/g dry weight from the control value of 21.6 μmol/g dry weight and tissue Ca2+ content was increased threefold. The treatment with Superoxide dismutase (SOD) and catalase (CAT) increased percentage of normal cells (62.2%) compared to nontreated Ca2+ paradox group (0.2%) and caused negligible leakage of CPK. Tissue ATP was preserved (p<0.03), and Ca2+ content was also reduced in the hearts treated with SOD and CAT (p<0.03). The cell membranes and vascular endothelium were well preserved in the hearts treated with SOD and CAT. Boiled SOD and CAT administered were totally ineffective. It is suggested that oxygen-active species may have a role in the Ca2+ paradox injury.

Original languageEnglish (US)
Pages (from-to)27-37
Number of pages11
JournalVirchows Archiv B Cell Pathology Including Molecular Pathology
Volume54
Issue number1
DOIs
StatePublished - Dec 1 1987
Externally publishedYes

Fingerprint

Heart Injuries
Catalase
Superoxide Dismutase
Reactive Oxygen Species
Calcium
Creatine Kinase
Adenosine Triphosphate
Weights and Measures
Cell Separation
Vascular Endothelium
Endothelial Cells
Perfusion
Cell Membrane
Wounds and Injuries
Enzymes

Keywords

  • ATP
  • Ca
  • Calcium paradox
  • Catalase
  • Cell membrane
  • Cytochrome C
  • Heart
  • Oxygen derived radicals
  • Superoxide anion
  • Superoxide dismutase
  • Vascular endothelium

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

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title = "Oxygen derived radicals related injury in the heart during calcium paradox",
abstract = "The effects of oxygen-derived radical scavengers (ODRS) on the heart was investigated during the calcium paradox. Perfusion with Ca2 +-free medium caused cell separation at the intercalated discs and changes in the endothelial cells. Upon Ca2+ reintroduction, a massive cell damage occurred. The cytosolic enzyme, creatine phosphokinase (CPK), was released in large amounts (p< 0.001). The tissue adenosine triphosphate (ATP) was reduced to 3.7 μmol/g dry weight from the control value of 21.6 μmol/g dry weight and tissue Ca2+ content was increased threefold. The treatment with Superoxide dismutase (SOD) and catalase (CAT) increased percentage of normal cells (62.2{\%}) compared to nontreated Ca2+ paradox group (0.2{\%}) and caused negligible leakage of CPK. Tissue ATP was preserved (p<0.03), and Ca2+ content was also reduced in the hearts treated with SOD and CAT (p<0.03). The cell membranes and vascular endothelium were well preserved in the hearts treated with SOD and CAT. Boiled SOD and CAT administered were totally ineffective. It is suggested that oxygen-active species may have a role in the Ca2+ paradox injury.",
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author = "Muhammad Ashraf",
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AB - The effects of oxygen-derived radical scavengers (ODRS) on the heart was investigated during the calcium paradox. Perfusion with Ca2 +-free medium caused cell separation at the intercalated discs and changes in the endothelial cells. Upon Ca2+ reintroduction, a massive cell damage occurred. The cytosolic enzyme, creatine phosphokinase (CPK), was released in large amounts (p< 0.001). The tissue adenosine triphosphate (ATP) was reduced to 3.7 μmol/g dry weight from the control value of 21.6 μmol/g dry weight and tissue Ca2+ content was increased threefold. The treatment with Superoxide dismutase (SOD) and catalase (CAT) increased percentage of normal cells (62.2%) compared to nontreated Ca2+ paradox group (0.2%) and caused negligible leakage of CPK. Tissue ATP was preserved (p<0.03), and Ca2+ content was also reduced in the hearts treated with SOD and CAT (p<0.03). The cell membranes and vascular endothelium were well preserved in the hearts treated with SOD and CAT. Boiled SOD and CAT administered were totally ineffective. It is suggested that oxygen-active species may have a role in the Ca2+ paradox injury.

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