P15INK4B gene methylation and expression in normal, myelodysplastic, and acute myelogenous leukemia cells and in the marrow cells of cured lymphoma patients

H. D. Preisler, Biaoru Li, H. Chen, L. Fisher, J. Nayini, A. Raza, S. Creech, P. Venugopal

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37 Scopus citations

Abstract

P15INK4B methylation and expression was studied in bone marrow cells obtained from normal individuals, from patients who had been cured of lymphoma, and from patients with either MDS or AML. The level of p15 methylation was very low in normal BM cells and in CD34+ and CD34 subpopulations (0-6.5%; med, = 2.5%). P15INK4B transcripts were present in each of these cell populations. In contrast, methylation was the usual situation in MDS and AML marrows. The presence of methylation of the p15INK4B gene did not always indicate an absence of expression nor was expression always present if methylation was absent. P15INK4B methylation was studied in the marrows of nine patients (one studied twice) who had been cured of lymphoma and in whom hemopoiesis was believed to be normal. Increased methylaton was present in all 10 marrows. These data indicate that p15INK4B methylation is likely to be a very early event in the development of the secondary hematologic disorders.

Original languageEnglish (US)
Pages (from-to)1589-1595
Number of pages7
JournalLeukemia
Volume15
Issue number10
DOIs
Publication statusPublished - Jan 1 2001

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Keywords

  • Gene methylation
  • P15
  • Proliferation control
  • Secondary hematologic disorders

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Cancer Research

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