Palmitate oxidation in human muscle

Comparison to CPT and carnitine

Jack B. Shumate, James Edwin Carroll, Michael H. Brooke, Rati M. Choksi

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The evaluation of palmitate oxidation in muscle tissue may be a useful screening test for detecting defects in fatty acid metabolism in human neuromuscular disease. If the test is to be useful, it is necessary to obtain data on a wide variety of muscle illnesses for comparative purposes. We report our experience with palmitate oxidation, muscle carnitine, and carnitine palmityl transferase (CPT) activity in 148 muscle biopsies from a variety of illnesses. The efficacy of using total protein, citrate synthase, and (1‐14C) pyruvate oxidation as internal references was investigated. Palmitate oxidation was significantly less than normal (P ≤ 0.01) in Duchenne muscular dystrophy, congenital nonprogressive myopathy, congenital muscular dystrophy, malignant hyperpyrexia, and denervation, depending on the internal reference used. Muscle carnitine levels followed a similar pattern, however, CPT activity did not. The possibility of these findings being secondary to inactivity is discussed.

Original languageEnglish (US)
Pages (from-to)226-231
Number of pages6
JournalMuscle & nerve
Volume5
Issue number3
DOIs
StatePublished - Jan 1 1982
Externally publishedYes

Fingerprint

Carnitine
Palmitates
Transferases
Muscles
Myotonia Congenita
Citrate (si)-Synthase
Malignant Hyperthermia
Neuromuscular Diseases
Duchenne Muscular Dystrophy
Muscular Dystrophies
Denervation
Pyruvic Acid
Fatty Acids
Biopsy
Proteins

ASJC Scopus subject areas

  • Physiology
  • Clinical Neurology
  • Cellular and Molecular Neuroscience
  • Physiology (medical)

Cite this

Palmitate oxidation in human muscle : Comparison to CPT and carnitine. / Shumate, Jack B.; Carroll, James Edwin; Brooke, Michael H.; Choksi, Rati M.

In: Muscle & nerve, Vol. 5, No. 3, 01.01.1982, p. 226-231.

Research output: Contribution to journalArticle

Shumate, Jack B. ; Carroll, James Edwin ; Brooke, Michael H. ; Choksi, Rati M. / Palmitate oxidation in human muscle : Comparison to CPT and carnitine. In: Muscle & nerve. 1982 ; Vol. 5, No. 3. pp. 226-231.
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