Par1b Induces Asymmetric Inheritance of Plasma Membrane Domains via LGN-Dependent Mitotic Spindle Orientation in Proliferating Hepatocytes

Christiaan L. Slim, Francisco Lázaro-Diéguez, Marjolein Bijlard, Mathilda J.M. Toussaint, Alain de Bruin, Quansheng Du, Anne Müsch, Sven C.D. van IJzendoorn

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The development and maintenance of polarized epithelial tissue requires a tightly controlled orientation of mitotic cell division relative to the apical polarity axis. Hepatocytes display a unique polarized architecture. We demonstrate that mitotic hepatocytes asymmetrically segregate their apical plasma membrane domain to the nascent daughter cells. The non-polarized nascent daughter cell can form a de novo apical domain with its new neighbor. This asymmetric segregation of apical domains is facilitated by a geometrically distinct "apicolateral" subdomain of the lateral surface present in hepatocytes. The polarity protein partitioning-defective 1/microtubule-affinity regulating kinase 2 (Par1b/MARK2) translates this positional landmark to cortical polarity by promoting the apicolateral accumulation of Leu-Gly-Asn repeat-enriched protein (LGN) and the capture of nuclear mitotic apparatus protein (NuMA)-positive astral microtubules to orientate the mitotic spindle. Proliferating hepatocytes thus display an asymmetric inheritance of their apical domains via a mechanism that involves Par1b and LGN, which we postulate serves the unique tissue architecture of the developing liver parenchyma.

Original languageEnglish (US)
Article numbere1001739
JournalPLoS Biology
Volume11
Issue number12
DOIs
StatePublished - Dec 1 2013

Fingerprint

mitotic spindle apparatus
Spindle Apparatus
Cell membranes
hepatocytes
Hepatocytes
inheritance (genetics)
plasma membrane
Cell Membrane
Microtubules
microtubules
Tissue
Proteins
proteins
Cell Division
Liver
cell division
phosphotransferases (kinases)
Phosphotransferases
epithelium
Epithelium

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Slim, C. L., Lázaro-Diéguez, F., Bijlard, M., Toussaint, M. J. M., de Bruin, A., Du, Q., ... van IJzendoorn, S. C. D. (2013). Par1b Induces Asymmetric Inheritance of Plasma Membrane Domains via LGN-Dependent Mitotic Spindle Orientation in Proliferating Hepatocytes. PLoS Biology, 11(12), [e1001739]. https://doi.org/10.1371/journal.pbio.1001739

Par1b Induces Asymmetric Inheritance of Plasma Membrane Domains via LGN-Dependent Mitotic Spindle Orientation in Proliferating Hepatocytes. / Slim, Christiaan L.; Lázaro-Diéguez, Francisco; Bijlard, Marjolein; Toussaint, Mathilda J.M.; de Bruin, Alain; Du, Quansheng; Müsch, Anne; van IJzendoorn, Sven C.D.

In: PLoS Biology, Vol. 11, No. 12, e1001739, 01.12.2013.

Research output: Contribution to journalArticle

Slim, CL, Lázaro-Diéguez, F, Bijlard, M, Toussaint, MJM, de Bruin, A, Du, Q, Müsch, A & van IJzendoorn, SCD 2013, 'Par1b Induces Asymmetric Inheritance of Plasma Membrane Domains via LGN-Dependent Mitotic Spindle Orientation in Proliferating Hepatocytes', PLoS Biology, vol. 11, no. 12, e1001739. https://doi.org/10.1371/journal.pbio.1001739
Slim, Christiaan L. ; Lázaro-Diéguez, Francisco ; Bijlard, Marjolein ; Toussaint, Mathilda J.M. ; de Bruin, Alain ; Du, Quansheng ; Müsch, Anne ; van IJzendoorn, Sven C.D. / Par1b Induces Asymmetric Inheritance of Plasma Membrane Domains via LGN-Dependent Mitotic Spindle Orientation in Proliferating Hepatocytes. In: PLoS Biology. 2013 ; Vol. 11, No. 12.
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