Parathyroid Hormone Stimulates Electrogenic Sodium Transport in A6 Cells

Jose Rodriguezcommes, John N. Forrest, Raquel Lopez, Jose Gasallaherraiz, Carlos M Isales

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The effects of parathyroid hormone (PTH) on sodium homeostasis in the distal tubule are not well defined. Using A6 cells as a model for distal tubular epithelium we measured equivalent short circuit current (leq), as an estimate of net sodium transport. We found that PTH increased leq in a dose-dependent manner. DDA, an agent which inhibits adenylate cyclase, decreased PTH-activated sodium transport, suggesting a role for cAMP elevation in PTH effects. Moreover, addition of Rp-cAMP, an inhibitor of cAMP-dependent protein kinase, partially blocked the PTH-stimulated leq. PTH also elicited a sustained increase in [Ca2+]i in A6 cells. This elevation in [Ca2+]i was abolished by removal of calcium from the extracellular medium, suggesting the involvement of calcium influx pathways. In fact, addition of the calcium channel blocker nitrendipine to PTH-stimulated leq partially blocked PTH-activated sodium transport. Taken together these data demonstrate that PTH stimulates electrogenic sodium transport in A6 cells and that this effect may be mediated through a rise in both intracellular calcium and cellular cAMP.

Original languageEnglish (US)
Pages (from-to)688-698
Number of pages11
JournalBiochemical and Biophysical Research Communications
Volume213
Issue number2
DOIs
StatePublished - Jan 1 1995

Fingerprint

Parathyroid Hormone
Sodium
Calcium
Nitrendipine
Calcium Channel Blockers
Cyclic AMP-Dependent Protein Kinases
Adenylyl Cyclases
Short circuit currents
Homeostasis
Epithelium

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Parathyroid Hormone Stimulates Electrogenic Sodium Transport in A6 Cells. / Rodriguezcommes, Jose; Forrest, John N.; Lopez, Raquel; Gasallaherraiz, Jose; Isales, Carlos M.

In: Biochemical and Biophysical Research Communications, Vol. 213, No. 2, 01.01.1995, p. 688-698.

Research output: Contribution to journalArticle

Rodriguezcommes, Jose ; Forrest, John N. ; Lopez, Raquel ; Gasallaherraiz, Jose ; Isales, Carlos M. / Parathyroid Hormone Stimulates Electrogenic Sodium Transport in A6 Cells. In: Biochemical and Biophysical Research Communications. 1995 ; Vol. 213, No. 2. pp. 688-698.
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