PEG-stabilized carbodiimide crosslinked collagen-chitosan hydrogels for corneal tissue engineering

Mehrdad Rafat, Fengfu Li, Per Fagerholm, Neil S. Lagali, Mitchell Aaron Watsky, Rejean Munger, Takeshi Matsuura, May Griffith

Research output: Contribution to journalArticle

238 Citations (Scopus)

Abstract

Implantable biomaterials that mimic the extracellular matrix (ECM) in key physical and physiological functions require components and microarchitectures that are carefully designed to maintain the correct balance between biofunctional and physical properties. Our goal was to develop hybrid polymer networks (HPN) that combine the bioactive features of natural materials and physical characteristics of synthetic ones to achieve synergy between the desirable mechanical properties of some components with the biological compatibility and physiological relevance of others. In this study, we developed collagen-chitosan composite hydrogels as corneal implants stabilized by either a simple carbodiimide cross-linker or a hybrid cross-linking system comprised of a long-range bi-functional cross-linker (e.g. poly(ethylene glycol) dibutyraldehyde (PEG-DBA)), and short-range amide-type cross-linkers (e.g. 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), and N-hydroxysuccinimide (NHS)). Optimum hybrid hydrogel demonstrated significantly enhanced mechanical strength and elasticity by 100 and 20%, respectively, compared to its non-hybrid counterpart. It demonstrated excellent optical properties, optimum mechanical properties and suturability, and good permeability to glucose and albumin. It had excellent biocompatibility and when implanted into pig corneas for 12 months, allowed seamless host-graft integration with successful regeneration of host corneal epithelium, stroma, and nerves.

Original languageEnglish (US)
Pages (from-to)3960-3972
Number of pages13
JournalBiomaterials
Volume29
Issue number29
DOIs
StatePublished - Oct 1 2008
Externally publishedYes

Fingerprint

Carbodiimides
Hydrogels
Chitosan
Tissue Engineering
Tissue engineering
Collagen
Polyethylene glycols
Ethyldimethylaminopropyl Carbodiimide
Corneal Stroma
Mechanical properties
Corneal Epithelium
Ethylene Glycol
Hydrogel
Elasticity
Biocompatible Materials
Biocompatibility
Amides
Biomaterials
Grafts
Cornea

Keywords

  • Biomimetic material
  • Collagen
  • Confocal microscopy
  • Cornea
  • Cross-linking
  • Nerve regeneration

ASJC Scopus subject areas

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

Cite this

PEG-stabilized carbodiimide crosslinked collagen-chitosan hydrogels for corneal tissue engineering. / Rafat, Mehrdad; Li, Fengfu; Fagerholm, Per; Lagali, Neil S.; Watsky, Mitchell Aaron; Munger, Rejean; Matsuura, Takeshi; Griffith, May.

In: Biomaterials, Vol. 29, No. 29, 01.10.2008, p. 3960-3972.

Research output: Contribution to journalArticle

Rafat, M, Li, F, Fagerholm, P, Lagali, NS, Watsky, MA, Munger, R, Matsuura, T & Griffith, M 2008, 'PEG-stabilized carbodiimide crosslinked collagen-chitosan hydrogels for corneal tissue engineering', Biomaterials, vol. 29, no. 29, pp. 3960-3972. https://doi.org/10.1016/j.biomaterials.2008.06.017
Rafat, Mehrdad ; Li, Fengfu ; Fagerholm, Per ; Lagali, Neil S. ; Watsky, Mitchell Aaron ; Munger, Rejean ; Matsuura, Takeshi ; Griffith, May. / PEG-stabilized carbodiimide crosslinked collagen-chitosan hydrogels for corneal tissue engineering. In: Biomaterials. 2008 ; Vol. 29, No. 29. pp. 3960-3972.
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