Phospholipase D activity underlies very-low-density lipoprotein (VLDL)-induced aldosterone production in adrenal glomerulosa cells

Ying Ying Tsai, William E. Rainey, Zhi Qiang Pan, Michael A. Frohman, Vivek Choudhary, Wendy B Bollag

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Aldosterone is the mineralocorticoid responsible for sodium retention, thus increased blood volume and pressure. Excessive production of aldosterone results in high blood pressure as well as renal disease, stroke, and visual loss via both direct effects and effects on blood pressure. Weight gain is often associated with increased blood pressure, but it remains unclearhowobesity increases blood pressure. Obese patients typically have higher lipoprotein levels; moreover, some studies have suggested that aldosterone levels are also elevated and represent a link between obesity and hypertension. Very-low-density lipoprotein (VLDL) functions to transport triglycerides from the liver to peripheral tissues. Although previous studies have demonstrated that VLDL can stimulate aldosterone production, the mechanisms underlying this effect are largely unclear. Here we show for the first time that phospholipase D (PLD) is involved in VLDL-induced aldosterone production in both a human adrenocortical cell line (HAC15) and primary cultures of bovine zona glomerulosa cells. Our data also reveal that PLD mediates steroidogenic acute regulatory (StAR) protein and aldosterone synthase (CYP11B2) expression via increasing the phosphorylation (activation) of their regulatory transcription factors. Finally, by using selective PLD inhibitors, our studies suggest that both PLD1 and PLD2 isoforms play an important role in VLDL-induced aldosterone production. Copyright

Original languageEnglish (US)
Pages (from-to)3550-3560
Number of pages11
JournalEndocrinology
Volume155
Issue number9
DOIs
StatePublished - Sep 1 2014

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Zona Glomerulosa
Phospholipase D
VLDL Lipoproteins
Aldosterone
Cytochrome P-450 CYP11B2
Blood Pressure
Hypertension
Mineralocorticoids
Blood Volume
Lipoproteins
Weight Gain
Protein Isoforms
Triglycerides
Transcription Factors
Obesity
Sodium
Stroke
Phosphorylation
Kidney
Cell Line

ASJC Scopus subject areas

  • Endocrinology

Cite this

Phospholipase D activity underlies very-low-density lipoprotein (VLDL)-induced aldosterone production in adrenal glomerulosa cells. / Tsai, Ying Ying; Rainey, William E.; Pan, Zhi Qiang; Frohman, Michael A.; Choudhary, Vivek; Bollag, Wendy B.

In: Endocrinology, Vol. 155, No. 9, 01.09.2014, p. 3550-3560.

Research output: Contribution to journalArticle

Tsai, Ying Ying ; Rainey, William E. ; Pan, Zhi Qiang ; Frohman, Michael A. ; Choudhary, Vivek ; Bollag, Wendy B. / Phospholipase D activity underlies very-low-density lipoprotein (VLDL)-induced aldosterone production in adrenal glomerulosa cells. In: Endocrinology. 2014 ; Vol. 155, No. 9. pp. 3550-3560.
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