Physical and genetic mapping of the telomeric major histocompatibility complex region in man and relevance to the primary hemochromatosis gene (HFE)

Jeffrey R. Gruen, Vita Lan-Ing Goei, Kim M. Summers, Angela Capossela, Lawrie Powell, June Halliday, Huda Zoghbi, Hridayabhiranjan Shukla, Sherman M. Weissman

Research output: Contribution to journalArticle

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Abstract

We performed pulsed-field gel electrophoresis (PFGE) on genomic DNA from a radiation hybrid (RH) cell line and constructed a high-resolution physical map of the major histocompatibility complex class I region in 6p21.3, where the gene for primary hemochromatosis (HFE) is believed to be located. Due to the intact microsegment of hemizygous human genomic DNA preserved in the RH cell line, simplified and distinct restriction fragment banding patterns were generated. Using the RH cell line, we were able to extend the physical map of the HLA class I region to about 3000 kb, order the known HLA class I genes from centromere to telomere: HLA-B, -C, -E, (-A, -H, -G), and -F, and orient the HLA-F gene along the chromosome. The proximity of HLA-F to HLA-A was confirmed by linkage and linkage disequilibrium analysis. This study shows that RH cell lines can be useful for constructing long-range physical maps in specific regions of the human genome with PFGE. Physical and genetic mapping studies of this region are consistent with a localization of the HFE gene proximal or distal to HLA-A.

Original languageEnglish (US)
Pages (from-to)232-240
Number of pages9
JournalGenomics
Volume14
Issue number2
DOIs
StatePublished - Jan 1 1992
Externally publishedYes

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Physical Chromosome Mapping
Radiation Hybrid Mapping
Hemochromatosis
Hybrid Cells
Major Histocompatibility Complex
Cell Line
HLA-A Antigens
Pulsed Field Gel Electrophoresis
Genes
HLA-C Antigens
MHC Class I Genes
HLA-B Antigens
Centromere
DNA
Telomere
Linkage Disequilibrium
Human Genome
Chromosomes

ASJC Scopus subject areas

  • Genetics

Cite this

Physical and genetic mapping of the telomeric major histocompatibility complex region in man and relevance to the primary hemochromatosis gene (HFE). / Gruen, Jeffrey R.; Goei, Vita Lan-Ing; Summers, Kim M.; Capossela, Angela; Powell, Lawrie; Halliday, June; Zoghbi, Huda; Shukla, Hridayabhiranjan; Weissman, Sherman M.

In: Genomics, Vol. 14, No. 2, 01.01.1992, p. 232-240.

Research output: Contribution to journalArticle

Gruen, Jeffrey R. ; Goei, Vita Lan-Ing ; Summers, Kim M. ; Capossela, Angela ; Powell, Lawrie ; Halliday, June ; Zoghbi, Huda ; Shukla, Hridayabhiranjan ; Weissman, Sherman M. / Physical and genetic mapping of the telomeric major histocompatibility complex region in man and relevance to the primary hemochromatosis gene (HFE). In: Genomics. 1992 ; Vol. 14, No. 2. pp. 232-240.
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