TY - JOUR
T1 - Physical and genetic mapping of the telomeric major histocompatibility complex region in man and relevance to the primary hemochromatosis gene (HFE)
AU - Gruen, Jeffrey R.
AU - Goei, Vita L.
AU - Summers, Kim M.
AU - Capossela, Angela
AU - Powell, Lawrie
AU - Halliday, June
AU - Zoghbi, Huda
AU - Shukla, Hridayabhiranjan
AU - Weissman, Sherman M.
N1 - Funding Information:
This work was supported by the Charles H. Hood Foundation, The Swebilius Cancer Research Fund, NIH Grants 5-P30-DK34989 (Digestive Diseases Research Core Center) and 1-P30-HD27757 (Chil-drens Health Research Center), and the National Health and Medical Research Council of Australia. The authors thank D. Geraghty and H. T. Orr for generously supplying the probe 5.4 SH and the plasmid pHLA5.4, and also R. Sarkar, J. R. Kidd, and K. K. Kidd for their helpful suggestions. J.R.G. wishes to thank Susan Gruen.
PY - 1992/10
Y1 - 1992/10
N2 - We performed pulsed-field gel electrophoresis (PFGE) on genomic DNA from a radiation hybrid (RH) cell line and constructed a high-resolution physical map of the major histocompatibility complex class I region in 6p21.3, where the gene for primary hemochromatosis (HFE) is believed to be located. Due to the intact microsegment of hemizygous human genomic DNA preserved in the RH cell line, simplified and distinct restriction fragment banding patterns were generated. Using the RH cell line, we were able to extend the physical map of the HLA class I region to about 3000 kb, order the known HLA class I genes from centromere to telomere: HLA-B, -C, -E, (-A, -H, -G), and -F, and orient the HLA-F gene along the chromosome. The proximity of HLA-F to HLA-A was confirmed by linkage and linkage disequilibrium analysis. This study shows that RH cell lines can be useful for constructing long-range physical maps in specific regions of the human genome with PFGE. Physical and genetic mapping studies of this region are consistent with a localization of the HFE gene proximal or distal to HLA-A.
AB - We performed pulsed-field gel electrophoresis (PFGE) on genomic DNA from a radiation hybrid (RH) cell line and constructed a high-resolution physical map of the major histocompatibility complex class I region in 6p21.3, where the gene for primary hemochromatosis (HFE) is believed to be located. Due to the intact microsegment of hemizygous human genomic DNA preserved in the RH cell line, simplified and distinct restriction fragment banding patterns were generated. Using the RH cell line, we were able to extend the physical map of the HLA class I region to about 3000 kb, order the known HLA class I genes from centromere to telomere: HLA-B, -C, -E, (-A, -H, -G), and -F, and orient the HLA-F gene along the chromosome. The proximity of HLA-F to HLA-A was confirmed by linkage and linkage disequilibrium analysis. This study shows that RH cell lines can be useful for constructing long-range physical maps in specific regions of the human genome with PFGE. Physical and genetic mapping studies of this region are consistent with a localization of the HFE gene proximal or distal to HLA-A.
UR - http://www.scopus.com/inward/record.url?scp=0026490373&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0026490373&partnerID=8YFLogxK
U2 - 10.1016/S0888-7543(05)80211-3
DO - 10.1016/S0888-7543(05)80211-3
M3 - Article
C2 - 1427838
AN - SCOPUS:0026490373
SN - 0888-7543
VL - 14
SP - 232
EP - 240
JO - Genomics
JF - Genomics
IS - 2
ER -