Pluripotency factors Lin28 and Oct4 identify a sub-population of stem cell-like cells in ovarian cancer

S. Peng, Nita Jane Maihle, Y. Huang

Research output: Contribution to journalArticle

186 Citations (Scopus)

Abstract

Lin28 and Oct4 are highly expressed in human embryonic stem (ES) cells and, along with two other stem cell marker proteins (Nanog and Sox2), together can convert human somatic cells to pluripotency. As an RNA-binding protein, Lin28 acts to stimulate the translation of a specific subset of mRNAs, and to inhibit the biogenesis of a group of microRNAs. Oct4 is a transcription factor essential for the maintenance of pluripotency and survival of ES cells. In this study, we report that a sub-population of epithelial ovarian cancer (EOC) cells co-expresses Lin28 and Oct4 as demonstrated in the analyses of both cell lines and patient tumor samples. We also observe that the combined expression of these proteins in tumor samples is correlated with advanced tumor grade. Intriguingly, when the expression of these two proteins is repressed in the same cells using RNA interference, there is significant reduction in cell growth and survival. We thus propose that Lin28 and Oct4 may have important roles in the initiation and/or progression of EOC, and consequently may serve as important molecular diagnostics and/or therapeutic targets for the development of novel treatment strategies in EOC patients.

Original languageEnglish (US)
Pages (from-to)2153-2159
Number of pages7
JournalOncogene
Volume29
Issue number14
DOIs
StatePublished - Apr 1 2010

Fingerprint

Ovarian Neoplasms
Stem Cells
Population
RNA-Binding Proteins
Molecular Pathology
Embryonic Stem Cells
RNA Interference
Tumor Cell Line
MicroRNAs
Neoplasms
Cell Survival
Proteins
Transcription Factors
Maintenance
Messenger RNA
Therapeutics
Growth
Ovarian epithelial cancer
Nanog Homeobox Protein
Human Embryonic Stem Cells

Keywords

  • Cancer stem cell
  • Gene therapy
  • Lin28
  • Molecular diagnostics
  • Oct4
  • Ovarian cancer

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

Cite this

Pluripotency factors Lin28 and Oct4 identify a sub-population of stem cell-like cells in ovarian cancer. / Peng, S.; Maihle, Nita Jane; Huang, Y.

In: Oncogene, Vol. 29, No. 14, 01.04.2010, p. 2153-2159.

Research output: Contribution to journalArticle

Peng, S. ; Maihle, Nita Jane ; Huang, Y. / Pluripotency factors Lin28 and Oct4 identify a sub-population of stem cell-like cells in ovarian cancer. In: Oncogene. 2010 ; Vol. 29, No. 14. pp. 2153-2159.
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