Polyamines in cultured rabbit corneal cells

Haiming Du, Mary Jane Viar, Leonard R. Johnson, Mitchell Aaron Watsky

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

PURPOSE. To determine whether polyamines are present in corneal cells, whether corneal cell polyamines can be depleted by blocking the first rate-limiting enzyme in the polyamine synthesis pathway, ornithine decarboxylase (ODC), and whether polyamines are required for proliferation in all three corneal cell types. METHODS. Cultured corneal epithelial cells, keratocytes, and endothelial cells were exposed to the specific ODC blocker difluoromethylornithine (DFMO), and ODC activity, intracellular polyamine concentrations, and cell proliferation were measured. RESULTS. DFMO blocked ODC activity in a dose- and time-dependent manner in all three cell types. DFMO treatment completely depleted putrescine and spermidine by 2 days and also significantly depleted spermine. DFMO treatment also inhibited cell growth in all three cell types and this inhibition could be completely reversed by adding exogenous putrescine to the culture medium. CONCLUSIONS. Polyamines are present in all cell types of the cornea, their formation is catalyzed at least in part by ODC, and they are an important component of corneal cell proliferation.

Original languageEnglish (US)
Pages (from-to)2512-2517
Number of pages6
JournalInvestigative Ophthalmology and Visual Science
Volume44
Issue number6
DOIs
StatePublished - Jun 1 2003
Externally publishedYes

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Polyamines
Ornithine Decarboxylase
Eflornithine
Rabbits
Putrescine
Cell Proliferation
Spermidine
Spermine
Cornea
Culture Media
Endothelial Cells
Epithelial Cells
Enzymes
Growth

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Polyamines in cultured rabbit corneal cells. / Du, Haiming; Viar, Mary Jane; Johnson, Leonard R.; Watsky, Mitchell Aaron.

In: Investigative Ophthalmology and Visual Science, Vol. 44, No. 6, 01.06.2003, p. 2512-2517.

Research output: Contribution to journalArticle

Du, Haiming ; Viar, Mary Jane ; Johnson, Leonard R. ; Watsky, Mitchell Aaron. / Polyamines in cultured rabbit corneal cells. In: Investigative Ophthalmology and Visual Science. 2003 ; Vol. 44, No. 6. pp. 2512-2517.
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N2 - PURPOSE. To determine whether polyamines are present in corneal cells, whether corneal cell polyamines can be depleted by blocking the first rate-limiting enzyme in the polyamine synthesis pathway, ornithine decarboxylase (ODC), and whether polyamines are required for proliferation in all three corneal cell types. METHODS. Cultured corneal epithelial cells, keratocytes, and endothelial cells were exposed to the specific ODC blocker difluoromethylornithine (DFMO), and ODC activity, intracellular polyamine concentrations, and cell proliferation were measured. RESULTS. DFMO blocked ODC activity in a dose- and time-dependent manner in all three cell types. DFMO treatment completely depleted putrescine and spermidine by 2 days and also significantly depleted spermine. DFMO treatment also inhibited cell growth in all three cell types and this inhibition could be completely reversed by adding exogenous putrescine to the culture medium. CONCLUSIONS. Polyamines are present in all cell types of the cornea, their formation is catalyzed at least in part by ODC, and they are an important component of corneal cell proliferation.

AB - PURPOSE. To determine whether polyamines are present in corneal cells, whether corneal cell polyamines can be depleted by blocking the first rate-limiting enzyme in the polyamine synthesis pathway, ornithine decarboxylase (ODC), and whether polyamines are required for proliferation in all three corneal cell types. METHODS. Cultured corneal epithelial cells, keratocytes, and endothelial cells were exposed to the specific ODC blocker difluoromethylornithine (DFMO), and ODC activity, intracellular polyamine concentrations, and cell proliferation were measured. RESULTS. DFMO blocked ODC activity in a dose- and time-dependent manner in all three cell types. DFMO treatment completely depleted putrescine and spermidine by 2 days and also significantly depleted spermine. DFMO treatment also inhibited cell growth in all three cell types and this inhibition could be completely reversed by adding exogenous putrescine to the culture medium. CONCLUSIONS. Polyamines are present in all cell types of the cornea, their formation is catalyzed at least in part by ODC, and they are an important component of corneal cell proliferation.

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