Polyclonal antisera specific for the proenzyme form of each calpain

Dorothy E. Croall, Clive A. Slaughter, Helen S. Wortham, Colleen M. Skelly, Lynn DeOgny, Carolyn R. Moomaw

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Each subunit of calpain (EC 3.4.22.17) is proteolytically modified when the enzymes are exposed to calcium. These cleavages appear to be important for regulating the proteolytic activity and calcium-sensitivity of the proteinases. We have synthesized peptides that correspond to the sites of autoproteolytic modification within the catalytic subunit of each calpain. Polyclonal antisera raised against these peptides are highly specific for the unmodified catalytic subunit of each calpain. The antiserum specific for the N-terminal epitope of milli-calpain was used to demonstrate an inverse relationship between the presence of this N-terminal peptide and casein hydrolysis. The antiserum specific for the N-terminal epitope of micro-calpain was used to demonstrate proteolytic modification of the catalytic subunit of μ-calpain in rat erythrocytes treated with ionomycin and calcium.

Original languageEnglish (US)
Pages (from-to)47-53
Number of pages7
JournalBiochimica et Biophysica Acta (BBA)/Protein Structure and Molecular
Volume1121
Issue number1-2
DOIs
StatePublished - May 22 1992
Externally publishedYes

Fingerprint

Enzyme Precursors
Calpain
Immune Sera
Catalytic Domain
Calcium
Peptides
Epitopes
Ionomycin
Caseins
Rats
Hydrolysis
Peptide Hydrolases
Erythrocytes
Enzymes

Keywords

  • Calpain
  • Immunoblotting
  • Proteinase
  • Proteolysis

ASJC Scopus subject areas

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Molecular Biology

Cite this

Polyclonal antisera specific for the proenzyme form of each calpain. / Croall, Dorothy E.; Slaughter, Clive A.; Wortham, Helen S.; Skelly, Colleen M.; DeOgny, Lynn; Moomaw, Carolyn R.

In: Biochimica et Biophysica Acta (BBA)/Protein Structure and Molecular, Vol. 1121, No. 1-2, 22.05.1992, p. 47-53.

Research output: Contribution to journalArticle

Croall, Dorothy E. ; Slaughter, Clive A. ; Wortham, Helen S. ; Skelly, Colleen M. ; DeOgny, Lynn ; Moomaw, Carolyn R. / Polyclonal antisera specific for the proenzyme form of each calpain. In: Biochimica et Biophysica Acta (BBA)/Protein Structure and Molecular. 1992 ; Vol. 1121, No. 1-2. pp. 47-53.
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