Polymorphonuclear leucocytes as potential source of free radicals in the ischaemic-reperfused myocardium

D. Bagchi, D. K. Das, R. M. Engelman, M. R. Prasad, Ramiah Subramanian

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

The feasibility of polymorphonuclear leucocytes as a potential source of free radicals during reperfusion of ischaemic myocardium was evaluated. Isolated rat heart was perfused in the presence of f-Met-Leu-Phe-activated and normal polymorphonuclear leucocytes for 30min. To Judge the degree of cellular injury which might result from activated polymorphonuclear leucocytes during per fusion, isolated hearts were also perfused with superoxide onions, hydroxyl radicals, and hypochlorous acid-generating systems in the absence or presence of their corresponding scavengers, superoxide dismutase plus catalase, dimethylthiourea, and allo-purinol, respectively. Activated polymorphonuclear leucocytes stimulated the release of lactate dehydro-genase, a biological marker of cellular injury, and malondialdehyde, a presumptive marker for lipid peroxidation; increased tissue injury, as evidenced by morphologic examinations using light and electron microscopy; decreased dryjwet ratios of heart, signifying oedema formation; and reduced myocardial adenosine triphosphate and creatine phosphate content as well as coronary flow, indicating decreased myocardial performance. These biological, physiological, and morphologic parameters were reversed significantly, but not completely, by treating the heart with scavengers, superoxide dismutase plus catalase or allopurinol, but were reversed completely by simultaneous treatment with superoxide dismutase, catalase, and allopurinol. Comparable results were obtained when the hearts were treated with each of these free radical-generating systems and their corresponding scavengers. Generation of free radicals was confirmed either by cytochrome c reduction or by examining the chemiluminescence response using a luminometer. These results indicate that activated polymorphonuclear leucocytes can cause myocardial cellular injury equivalent to the damage caused by free radicals and oxidants which are present in an ischaemic-reperfused heart, suggesting that polymorphonuclear leucocytes may be a potential source of free radicals in the reperf used heart.

Original languageEnglish (US)
Pages (from-to)800-813
Number of pages14
JournalEuropean Heart Journal
Volume11
Issue number9
DOIs
StatePublished - Jan 1 1990

Fingerprint

Free Radicals
Myocardium
Neutrophils
Allopurinol
Catalase
Superoxide Dismutase
Wounds and Injuries
methionyl-leucyl-phenylalanine
Hypochlorous Acid
Onions
Phosphocreatine
Adenine Nucleotides
Luminescence
Cytochromes c
Malondialdehyde
Oxidants
Superoxides
Hydroxyl Radical
Lipid Peroxidation
Reperfusion

Keywords

  • Free radical
  • Heart
  • Ischaemia
  • Polymorphonuclear leucocytes
  • Reperfusion injury

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Polymorphonuclear leucocytes as potential source of free radicals in the ischaemic-reperfused myocardium. / Bagchi, D.; Das, D. K.; Engelman, R. M.; Prasad, M. R.; Subramanian, Ramiah.

In: European Heart Journal, Vol. 11, No. 9, 01.01.1990, p. 800-813.

Research output: Contribution to journalArticle

Bagchi, D. ; Das, D. K. ; Engelman, R. M. ; Prasad, M. R. ; Subramanian, Ramiah. / Polymorphonuclear leucocytes as potential source of free radicals in the ischaemic-reperfused myocardium. In: European Heart Journal. 1990 ; Vol. 11, No. 9. pp. 800-813.
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