Presence of gonadotropin-releasing hormone (GnRH) receptor mRNA in rat myenteric plexus cells

John S. Ho, Gregg T. Nagle, John R. Mathias, Mary H. Clench, Xuemo Fan, Gulgun D. Kalmaz, Judith E. Sallustio, Ervin Y. Eaker

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

Idiopathic neuromuscular disease of the gastrointestinal tract (functional bowel disease) is thought to result from the malfunction of neurons within the enteric nervous system. Gonadotropin-releasing hormone (GnRH) analogs have recently been shown to organize the disordered motility patterns typical in these patients and to produce significant, long-term symptomatic improvement. To determine whether GnRH analogs might bind to an endogenous enteric nervous system GnRH receptor, reverse transcription-polymerase chain reaction (RT-PCR) was performed using cultured neonatal rat enteric neuron RNA and rat GnRH receptor primers. A PCR product of the predicted size was cloned and nucleotide sequence analysis demonstrated that the myenteric plexus PCR product encoded a portion of the GnRH receptor sequence previously identified in rat pituitary. These results suggest that cells in the myenteric plexus express GnRH receptors that may bind exogenously administered GnRH analogs. The expression of GnRH receptors in enteric neurons would provide an explanation for the effectiveness of GnRH analogs in treatment of idiopathic neuromuscular disease of the gastrointestinal tract.

Original languageEnglish (US)
Pages (from-to)817-821
Number of pages5
JournalComparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
Volume113
Issue number4
DOIs
Publication statusPublished - Jan 1 1996
Externally publishedYes

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Keywords

  • GnRH analogs
  • GnRH native forms
  • GnRH receptor
  • enteric nerves
  • functional bowel disease
  • leuprolide acetate
  • myenteric neuron culture
  • reverse transcriptase-polymerase chain reaction

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Molecular Biology

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