Prevention of calcium paradox-related myocardial cell injury with diltiazem, a calcium channel blocking agent

Muhammad Ashraf, Masahiko Onda, John B. Benedict, Ronald W. Millard

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The effect of diltiazem on creatine kinase release and tissue adenosine triphosphate content was investigated during calcium paradox in the isolated perfused rat heart. Creatine kinase loss was minimal during the calcium-free phase, but there was a 100-fold increase in creatine kinase release after reperfusion with normal calcium-containing medium. Diltiazem reduced creatine kinase loss by 35 percent when added to calcium-free medium and by approximately 80 percent when added to both calcium-free and reperfusion media. Adenosine triphosphate content was significantly increased from 2.98 μmol in untreated calcium paradox hearts to 5 μmol/g dry weight in diltiazem-treated hearts. With hyopthermia the calcium paradox injury was completely inhibited if the temperature of calcium-free perfusion was maintained at 15 ° C. Diltiazem appears to exert its protective effect through its ability to prevent the cellular separation and alterations in the gap junctions during calcium deprivation of cells and to limit calcium entry into the cells after reperfusion with calcium-containing medium.

Original languageEnglish (US)
Pages (from-to)1675-1681
Number of pages7
JournalThe American Journal of Cardiology
Volume49
Issue number7
DOIs
StatePublished - Jan 1 1982
Externally publishedYes

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Diltiazem
Calcium Channels
Calcium
Wounds and Injuries
Creatine Kinase
Reperfusion
Adenosine Triphosphate
Gap Junctions
Perfusion
Weights and Measures

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Prevention of calcium paradox-related myocardial cell injury with diltiazem, a calcium channel blocking agent. / Ashraf, Muhammad; Onda, Masahiko; Benedict, John B.; Millard, Ronald W.

In: The American Journal of Cardiology, Vol. 49, No. 7, 01.01.1982, p. 1675-1681.

Research output: Contribution to journalArticle

Ashraf, Muhammad ; Onda, Masahiko ; Benedict, John B. ; Millard, Ronald W. / Prevention of calcium paradox-related myocardial cell injury with diltiazem, a calcium channel blocking agent. In: The American Journal of Cardiology. 1982 ; Vol. 49, No. 7. pp. 1675-1681.
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