Abstract
We report here on the primary structure and functional characteristics of the protein responsible for the system A amino acid transport activity that is known to be expressed in most human tissues. This transporter, designated ATA2 for amino acid transporter A2, was cloned from the human hepatoma cell line HepG2. Human ATA2 (hATA2) consists of 506 amino acids and exhibits a high degree of homology to rat ATA2. hATA2-specific mRNA is ubiquitously expressed in human tissues. When expressed in mammalian cells, hATA2 mediates Na+-dependent transport of α-(methylamino)isobutyric acid, a specific model substrate for system A. The transporter is specific for neutral amino acids. It is pH-sensitive and Li+-intolerant. The Na+:amino acid stoichiometry is 1:1. (C) 2000 Elsevier Science B.V.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1-6 |
| Number of pages | 6 |
| Journal | Biochimica et Biophysica Acta - Biomembranes |
| Volume | 1467 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jul 31 2000 |
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Keywords
- Amino acid transport
- Human
- Primary structure
- Stoichiometry
- Substrate specificity
- System A
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Cell Biology
Cite this
Primary structure, functional characteristics and tissue expression pattern of human ATA2, a subtype of amino acid transport system A. / Hatanaka, Takahiro; Huang, Wei; Wang, Haiping; Sugawara, Mitsuru; Prasad, Puttur D; Leibach, Frederick H.; Ganapathy, Vadivel.
In: Biochimica et Biophysica Acta - Biomembranes, Vol. 1467, No. 1, 31.07.2000, p. 1-6.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Primary structure, functional characteristics and tissue expression pattern of human ATA2, a subtype of amino acid transport system A
AU - Hatanaka, Takahiro
AU - Huang, Wei
AU - Wang, Haiping
AU - Sugawara, Mitsuru
AU - Prasad, Puttur D
AU - Leibach, Frederick H.
AU - Ganapathy, Vadivel
PY - 2000/7/31
Y1 - 2000/7/31
N2 - We report here on the primary structure and functional characteristics of the protein responsible for the system A amino acid transport activity that is known to be expressed in most human tissues. This transporter, designated ATA2 for amino acid transporter A2, was cloned from the human hepatoma cell line HepG2. Human ATA2 (hATA2) consists of 506 amino acids and exhibits a high degree of homology to rat ATA2. hATA2-specific mRNA is ubiquitously expressed in human tissues. When expressed in mammalian cells, hATA2 mediates Na+-dependent transport of α-(methylamino)isobutyric acid, a specific model substrate for system A. The transporter is specific for neutral amino acids. It is pH-sensitive and Li+-intolerant. The Na+:amino acid stoichiometry is 1:1. (C) 2000 Elsevier Science B.V.
AB - We report here on the primary structure and functional characteristics of the protein responsible for the system A amino acid transport activity that is known to be expressed in most human tissues. This transporter, designated ATA2 for amino acid transporter A2, was cloned from the human hepatoma cell line HepG2. Human ATA2 (hATA2) consists of 506 amino acids and exhibits a high degree of homology to rat ATA2. hATA2-specific mRNA is ubiquitously expressed in human tissues. When expressed in mammalian cells, hATA2 mediates Na+-dependent transport of α-(methylamino)isobutyric acid, a specific model substrate for system A. The transporter is specific for neutral amino acids. It is pH-sensitive and Li+-intolerant. The Na+:amino acid stoichiometry is 1:1. (C) 2000 Elsevier Science B.V.
KW - Amino acid transport
KW - Human
KW - Primary structure
KW - Stoichiometry
KW - Substrate specificity
KW - System A
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UR - http://www.scopus.com/inward/citedby.url?scp=0034738568&partnerID=8YFLogxK
U2 - 10.1016/S0005-2736(00)00252-2
DO - 10.1016/S0005-2736(00)00252-2
M3 - Article
C2 - 10930503
AN - SCOPUS:0034738568
VL - 1467
SP - 1
EP - 6
JO - Biochimica et Biophysica Acta - Biomembranes
JF - Biochimica et Biophysica Acta - Biomembranes
SN - 0005-2736
IS - 1
ER -