Production and characterization of a monoclonal antibody able to discriminate galectin-1 from galectin-2 and galectin-3

Jean Denis Cornillot, Michel Pontet, Catherine Dupuy, Ahmed Chadli, Michel Caron, Raymonde Joubert-Caron, Philippe Bourin, Dominique Bladier

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Antisera raised against galectin-1 exhibit crossreactivities with other galectins or related molecules. In order to overcome this problem, 3 monoclonal antibody to human brain galectin-1 was obtained by selecting clones without reactivity toward galectin-3. This mAb specifically bound galectin-1 of various animal origins but neither galectin-2 nor galectin-3. Western-blotting analysis of soluble human brain extracts after 2D gel electrophoresis revealed only the two most acidic isoforms of galectin-1. The ability of this mAb to bind galectin-1-asialofetuin complexes indicates that its epitope is not localized in galacarbohydrate recognition domain of galectin-1. This particularity induces with efficiency its monospecificity.

Original languageEnglish (US)
Pages (from-to)425-432
Number of pages8
JournalGlycobiology
Volume8
Issue number5
DOIs
StatePublished - May 1 1998
Externally publishedYes

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Galectin 2
Galectin 1
Galectin 3
Monoclonal Antibodies
Brain
Galectins
Electrophoresis, Gel, Two-Dimensional
Immune Sera
Epitopes
Electrophoresis
Protein Isoforms
Clone Cells
Western Blotting
Animals
Gels
Molecules

Keywords

  • Galectin/monoclonal antibody/carbohydrate recognition domain

ASJC Scopus subject areas

  • Biochemistry

Cite this

Production and characterization of a monoclonal antibody able to discriminate galectin-1 from galectin-2 and galectin-3. / Cornillot, Jean Denis; Pontet, Michel; Dupuy, Catherine; Chadli, Ahmed; Caron, Michel; Joubert-Caron, Raymonde; Bourin, Philippe; Bladier, Dominique.

In: Glycobiology, Vol. 8, No. 5, 01.05.1998, p. 425-432.

Research output: Contribution to journalArticle

Cornillot, JD, Pontet, M, Dupuy, C, Chadli, A, Caron, M, Joubert-Caron, R, Bourin, P & Bladier, D 1998, 'Production and characterization of a monoclonal antibody able to discriminate galectin-1 from galectin-2 and galectin-3', Glycobiology, vol. 8, no. 5, pp. 425-432. https://doi.org/10.1093/glycob/8.5.425
Cornillot, Jean Denis ; Pontet, Michel ; Dupuy, Catherine ; Chadli, Ahmed ; Caron, Michel ; Joubert-Caron, Raymonde ; Bourin, Philippe ; Bladier, Dominique. / Production and characterization of a monoclonal antibody able to discriminate galectin-1 from galectin-2 and galectin-3. In: Glycobiology. 1998 ; Vol. 8, No. 5. pp. 425-432.
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