Production and characterization of a monoclonal antibody able to discriminate galectin-1 from galectin-2 and galectin-3

Jean Denis Cornillot, Michel Pontet, Catherine Dupuy, Ahmed Chadli, Michel Caron, Raymonde Joubert-Caron, Philippe Bourin, Dominique Bladier

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Antisera raised against galectin-1 exhibit crossreactivities with other galectins or related molecules. In order to overcome this problem, 3 monoclonal antibody to human brain galectin-1 was obtained by selecting clones without reactivity toward galectin-3. This mAb specifically bound galectin-1 of various animal origins but neither galectin-2 nor galectin-3. Western-blotting analysis of soluble human brain extracts after 2D gel electrophoresis revealed only the two most acidic isoforms of galectin-1. The ability of this mAb to bind galectin-1-asialofetuin complexes indicates that its epitope is not localized in galacarbohydrate recognition domain of galectin-1. This particularity induces with efficiency its monospecificity.

Original languageEnglish (US)
Pages (from-to)425-432
Number of pages8
JournalGlycobiology
Volume8
Issue number5
DOIs
StatePublished - May 1 1998
Externally publishedYes

Keywords

  • Galectin/monoclonal antibody/carbohydrate recognition domain

ASJC Scopus subject areas

  • Biochemistry

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    Cornillot, J. D., Pontet, M., Dupuy, C., Chadli, A., Caron, M., Joubert-Caron, R., Bourin, P., & Bladier, D. (1998). Production and characterization of a monoclonal antibody able to discriminate galectin-1 from galectin-2 and galectin-3. Glycobiology, 8(5), 425-432. https://doi.org/10.1093/glycob/8.5.425