Antisera raised against galectin-1 exhibit crossreactivities with other galectins or related molecules. In order to overcome this problem, 3 monoclonal antibody to human brain galectin-1 was obtained by selecting clones without reactivity toward galectin-3. This mAb specifically bound galectin-1 of various animal origins but neither galectin-2 nor galectin-3. Western-blotting analysis of soluble human brain extracts after 2D gel electrophoresis revealed only the two most acidic isoforms of galectin-1. The ability of this mAb to bind galectin-1-asialofetuin complexes indicates that its epitope is not localized in galacarbohydrate recognition domain of galectin-1. This particularity induces with efficiency its monospecificity.
- Galectin/monoclonal antibody/carbohydrate recognition domain
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