Progression of micturition dysfunction associated with the development of heart failure in rats: Model of overactive bladder

A. G. Mora, S. R. Furquim, S. P. Tartarotti, D. R. Andrade, S. C. Janussi, K. Krikorian, T. Rocha, C. F. Franco-Penteado, D. G. Priolli, Fernanda Priviero, M. A. Claudino

Research output: Contribution to journalArticle

Abstract

Heart failure (HF) has a strong association with the development of lower urinary tract symptoms, especially overactive bladder (OAB); although this condition remains poorly investigated. In this study, we assess the aortocaval fistula (ACF) model as a novel experimental model of micturition dysfunction, associated with HF, focused on the molecular and functional studies to evaluate the autonomic nervous system and urinary bladder remodeling. Male rats were submitted to ACF for HF induction. Echocardiography, cystometric, histomorphometry and molecular analysis, as well as concentration-response curves to carbachol and ATP and frequency-response curves to electrical field stimulation (EFS) were evaluated in Sham and HF (4- and 12-weeksendpoint) groups. Compared to SHAM, HF groups exhibited progressive increases in the left ventricle (LV) mass and fractional shortening which indicates cardiac dysfunction, although HF was characterized only after 12 weeks by the reduced ejection fraction. For micturition function, HF groups presented increased non-voiding contractions (NVC) and decreased bladder capacity; however, when comparing HF groups, these urinary parameters were significantly impaired over the weeks (12-weeks). The contractile responses induced by CCh, ATP and EFS were greater in detrusor muscle (DSM) from HF rats. mRNA expression for muscarinic receptors (M2 and M3) was higher in DSM only after 12 weeks of ACF, in addition to MMP9 and TGF-beta. Histomorphometric revealed increased urothelium thickness in both HF groups, whereas DSM thickness occurred only after 12 weeks. Thus, the ACF model induced cardiac dyfunction with progressive micturition dysfunction over the weeks, characterized by increased DSM contractile mechanisms as well as extracellular matrix remodeling in the urinary bladder, representing a useful tool to evaluate the OAB associated with HF.

Original languageEnglish (US)
Pages (from-to)107-116
Number of pages10
JournalLife sciences
Volume226
DOIs
StatePublished - Jun 1 2019

Fingerprint

Overactive Urinary Bladder
Urination
Rats
Heart Failure
Fistula
Muscle
Muscles
Urinary Bladder
Electric Stimulation
Adenosine Triphosphate
Muscarinic M3 Receptors
Muscarinic M2 Receptors
Echocardiography
Urothelium
Lower Urinary Tract Symptoms
Autonomic Nervous System
Neurology
Carbachol
Transforming Growth Factor beta
Frequency response

Keywords

  • Aortocaval fistula
  • Detrusor muscle
  • Heart failure
  • Micturition
  • Overactive bladder

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Mora, A. G., Furquim, S. R., Tartarotti, S. P., Andrade, D. R., Janussi, S. C., Krikorian, K., ... Claudino, M. A. (2019). Progression of micturition dysfunction associated with the development of heart failure in rats: Model of overactive bladder. Life sciences, 226, 107-116. https://doi.org/10.1016/j.lfs.2019.04.017

Progression of micturition dysfunction associated with the development of heart failure in rats : Model of overactive bladder. / Mora, A. G.; Furquim, S. R.; Tartarotti, S. P.; Andrade, D. R.; Janussi, S. C.; Krikorian, K.; Rocha, T.; Franco-Penteado, C. F.; Priolli, D. G.; Priviero, Fernanda; Claudino, M. A.

In: Life sciences, Vol. 226, 01.06.2019, p. 107-116.

Research output: Contribution to journalArticle

Mora, AG, Furquim, SR, Tartarotti, SP, Andrade, DR, Janussi, SC, Krikorian, K, Rocha, T, Franco-Penteado, CF, Priolli, DG, Priviero, F & Claudino, MA 2019, 'Progression of micturition dysfunction associated with the development of heart failure in rats: Model of overactive bladder', Life sciences, vol. 226, pp. 107-116. https://doi.org/10.1016/j.lfs.2019.04.017
Mora, A. G. ; Furquim, S. R. ; Tartarotti, S. P. ; Andrade, D. R. ; Janussi, S. C. ; Krikorian, K. ; Rocha, T. ; Franco-Penteado, C. F. ; Priolli, D. G. ; Priviero, Fernanda ; Claudino, M. A. / Progression of micturition dysfunction associated with the development of heart failure in rats : Model of overactive bladder. In: Life sciences. 2019 ; Vol. 226. pp. 107-116.
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AU - Andrade, D. R.

AU - Janussi, S. C.

AU - Krikorian, K.

AU - Rocha, T.

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