Progressive elimination of microinjected trehalose during mouse embryonic development

Ali Eroglu, Gloria Elliott, Diane L. Wright, Mehmet Toner, Thomas L. Toth

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Recently, sugars such as trehalose have been introduced into mammalian cells by overcoming the permeability barrier of cell membranes, and have provided improved tolerance against stresses associated with freezing and drying. However, the fate of the intracellular sugars has remained an open question. To address this issue, mouse oocytes were microinjected with 0.1 mol/l trehalose, and intracellular trehalose and glucose concentrations were determined during embryonic development using a high performance liquid chromatography and pulsed amperometric detection protocol. Trehalose was not detected in non-injected controls at any stage of development. In the microinjection group, the amount of intracellular trehalose progressively decreased as embryos developed. There was a corresponding increase in intracellular glucose concentration at the two-cell stage, suggesting cleavage of trehalose to two glucose molecules. In summary, this study presents a simple, highly sensitive protocol to determine intracellular sugars. The data reveal rapid elimination of microinjected trehalose during embryonic development. These findings have implications for designing osmolarity-optimized culture media for sugar-injected oocytes.

Original languageEnglish (US)
Article number1645
Pages (from-to)503-510
Number of pages8
JournalReproductive BioMedicine Online
Volume10
Issue number4
DOIs
StatePublished - Jan 1 2005

Fingerprint

Trehalose
Embryonic Development
Glucose
Oocytes
Cell Membrane Permeability
Microinjections
Osmolar Concentration
Freezing
Culture Media
Embryonic Structures
High Pressure Liquid Chromatography

Keywords

  • Cryopreservation
  • Freezing
  • Glucose
  • HPLC
  • Oocyte
  • Sugar
  • Trehalose

ASJC Scopus subject areas

  • Reproductive Medicine
  • Developmental Biology

Cite this

Progressive elimination of microinjected trehalose during mouse embryonic development. / Eroglu, Ali; Elliott, Gloria; Wright, Diane L.; Toner, Mehmet; Toth, Thomas L.

In: Reproductive BioMedicine Online, Vol. 10, No. 4, 1645, 01.01.2005, p. 503-510.

Research output: Contribution to journalArticle

Eroglu, Ali ; Elliott, Gloria ; Wright, Diane L. ; Toner, Mehmet ; Toth, Thomas L. / Progressive elimination of microinjected trehalose during mouse embryonic development. In: Reproductive BioMedicine Online. 2005 ; Vol. 10, No. 4. pp. 503-510.
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