Prolidase contamination in commercial bovine serum albumin

Vadivel Ganapathy, Manasses Fonteles, David H. Pashley, Frederick H. Leibach

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Bovine serum albumin from a number of commercial sources were screened for the presence or absence of peptidase contamination. Peptidase activity was monitored using various peptides as substrates. Two commercial preparations were found to have peptidase activity, and the enzyme was identified, on the basis of its substrate specificity, as prolidase (EC 3.4.14.9). The contaminating activity was in the order of 3-4 units/g albumin.

Original languageEnglish (US)
Pages (from-to)154-157
Number of pages4
JournalBBA - General Subjects
Volume719
Issue number1
DOIs
StatePublished - Oct 28 1982

Fingerprint

proline dipeptidase
Bovine Serum Albumin
Contamination
Peptide Hydrolases
Substrates
Substrate Specificity
Albumins
Peptides
Enzymes

Keywords

  • Prolidase contamination
  • Serum albumin

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

Cite this

Ganapathy, V., Fonteles, M., Pashley, D. H., & Leibach, F. H. (1982). Prolidase contamination in commercial bovine serum albumin. BBA - General Subjects, 719(1), 154-157. https://doi.org/10.1016/0304-4165(82)90319-1

Prolidase contamination in commercial bovine serum albumin. / Ganapathy, Vadivel; Fonteles, Manasses; Pashley, David H.; Leibach, Frederick H.

In: BBA - General Subjects, Vol. 719, No. 1, 28.10.1982, p. 154-157.

Research output: Contribution to journalArticle

Ganapathy, V, Fonteles, M, Pashley, DH & Leibach, FH 1982, 'Prolidase contamination in commercial bovine serum albumin', BBA - General Subjects, vol. 719, no. 1, pp. 154-157. https://doi.org/10.1016/0304-4165(82)90319-1
Ganapathy, Vadivel ; Fonteles, Manasses ; Pashley, David H. ; Leibach, Frederick H. / Prolidase contamination in commercial bovine serum albumin. In: BBA - General Subjects. 1982 ; Vol. 719, No. 1. pp. 154-157.
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