Objective: To study the effect of exogenous angiotensin II (Ang II) on the proliferation of cultured pulmonary artery smooth muscle cells (PASMCs) and its mechanisms. Methods: We adopted cultured PASMCs to observe the effect of exogenous Ang II on the proliferation of cultured PASMCs and its mechanisms. Results: Exogenous Ang II stimulated the proliferation of cultured PASMCs in a dose-dependent manner (10-10 - 10-5 mol·L -1). The proliferative effect of Ang II on PASMCs was markedly inhibited by calcium channel antagonist Verapamil, PKC inhibitor Staurosporine and Na+-K+ -ATPase inhibitor amiloride; the decrease rates in 3H-Tdr incorporation and cell numbers were 44.3%, 33.2%, 36.5% and 31.7%, 25.5%, 28.1%, respectively (P <0.01). Conclusion: Ang II significantly stimulates the proliferation of cultured PASMCs, and the activation of Ca2+ channel, PKC and Na+-K +-ATPase may be important intracellular signal transduction mechanisms.
|Original language||English (US)|
|Number of pages||3|
|Journal||Bulletin of Hunan Medical University|
|Publication status||Published - Aug 28 2002|
- Angiotensin II
- Protein kinase C
ASJC Scopus subject areas