Proliferative effect of angiotensin II on cultured PASMCs and its mechanisms

Hui Jun Liu, Li Cao, Tian Zheng Yu

Research output: Contribution to journalArticle

Abstract

Objective: To study the effect of exogenous angiotensin II (Ang II) on the proliferation of cultured pulmonary artery smooth muscle cells (PASMCs) and its mechanisms. Methods: We adopted cultured PASMCs to observe the effect of exogenous Ang II on the proliferation of cultured PASMCs and its mechanisms. Results: Exogenous Ang II stimulated the proliferation of cultured PASMCs in a dose-dependent manner (10-10 - 10-5 mol·L -1). The proliferative effect of Ang II on PASMCs was markedly inhibited by calcium channel antagonist Verapamil, PKC inhibitor Staurosporine and Na+-K+ -ATPase inhibitor amiloride; the decrease rates in 3H-Tdr incorporation and cell numbers were 44.3%, 33.2%, 36.5% and 31.7%, 25.5%, 28.1%, respectively (P <0.01). Conclusion: Ang II significantly stimulates the proliferation of cultured PASMCs, and the activation of Ca2+ channel, PKC and Na+-K +-ATPase may be important intracellular signal transduction mechanisms.

Original languageEnglish (US)
Pages (from-to)302-304
Number of pages3
JournalBulletin of Hunan Medical University
Volume27
Issue number4
Publication statusPublished - Aug 28 2002

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Keywords

  • Angiotensin II
  • PASMC
  • Proliferation
  • Protein kinase C

ASJC Scopus subject areas

  • Medicine(all)

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