Prostate specific antigen and prostatitis II. PSA production and release kinetics in vitro

Timothy D. Moon, Durwood Earnest Neal, Sanda Clejan

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Elevations in serum prostate specific antigen (PSA) levels in patients with prostatitis are well known, but the pathophysiologic mechanisms involved with this phenomenon are poorly understood. We have recently evaluated the effect of prostatitis on PSA levels in primates. This data demonstrated a rapid rise in PSA, which subsequently fell along the biological decay curve. This suggested a release of sequestered PSA. We evaluated the effect of infection upon PSA production for the prostatic adenocarcinoma cell line LNCaP. The cell line was determined to produce 9.6 ± 2.7 fg/cell/hr PSA with essentially linear kinetics. No effect was seen with dead bacteria, bacterial supernatant or complement upon the PSA production. Live E. coli had no effect for 4–8 hours at which time the cells sloughed and PSA production ceased. No release of stored PSA was seen. These data do not elucidate the reasons for increased serum PSA levels found with acute bacterial prostatitis, but indicate that it is not a storage phenomenon.

Original languageEnglish (US)
Pages (from-to)113-116
Number of pages4
JournalThe Prostate
Volume20
Issue number2
DOIs
StatePublished - Jan 1 1992

Fingerprint

Prostatitis
Prostate-Specific Antigen
In Vitro Techniques
Cell Line
Serum
Primates
Adenocarcinoma
Escherichia coli
Bacteria

Keywords

  • bacterial infection
  • cell line

ASJC Scopus subject areas

  • Oncology
  • Urology

Cite this

Prostate specific antigen and prostatitis II. PSA production and release kinetics in vitro. / Moon, Timothy D.; Neal, Durwood Earnest; Clejan, Sanda.

In: The Prostate, Vol. 20, No. 2, 01.01.1992, p. 113-116.

Research output: Contribution to journalArticle

@article{7d2eb389b3b846338b2769dfb0da8505,
title = "Prostate specific antigen and prostatitis II. PSA production and release kinetics in vitro",
abstract = "Elevations in serum prostate specific antigen (PSA) levels in patients with prostatitis are well known, but the pathophysiologic mechanisms involved with this phenomenon are poorly understood. We have recently evaluated the effect of prostatitis on PSA levels in primates. This data demonstrated a rapid rise in PSA, which subsequently fell along the biological decay curve. This suggested a release of sequestered PSA. We evaluated the effect of infection upon PSA production for the prostatic adenocarcinoma cell line LNCaP. The cell line was determined to produce 9.6 ± 2.7 fg/cell/hr PSA with essentially linear kinetics. No effect was seen with dead bacteria, bacterial supernatant or complement upon the PSA production. Live E. coli had no effect for 4–8 hours at which time the cells sloughed and PSA production ceased. No release of stored PSA was seen. These data do not elucidate the reasons for increased serum PSA levels found with acute bacterial prostatitis, but indicate that it is not a storage phenomenon.",
keywords = "bacterial infection, cell line",
author = "Moon, {Timothy D.} and Neal, {Durwood Earnest} and Sanda Clejan",
year = "1992",
month = "1",
day = "1",
doi = "10.1002/pros.2990200206",
language = "English (US)",
volume = "20",
pages = "113--116",
journal = "Prostate",
issn = "0270-4137",
publisher = "Wiley-Liss Inc.",
number = "2",

}

TY - JOUR

T1 - Prostate specific antigen and prostatitis II. PSA production and release kinetics in vitro

AU - Moon, Timothy D.

AU - Neal, Durwood Earnest

AU - Clejan, Sanda

PY - 1992/1/1

Y1 - 1992/1/1

N2 - Elevations in serum prostate specific antigen (PSA) levels in patients with prostatitis are well known, but the pathophysiologic mechanisms involved with this phenomenon are poorly understood. We have recently evaluated the effect of prostatitis on PSA levels in primates. This data demonstrated a rapid rise in PSA, which subsequently fell along the biological decay curve. This suggested a release of sequestered PSA. We evaluated the effect of infection upon PSA production for the prostatic adenocarcinoma cell line LNCaP. The cell line was determined to produce 9.6 ± 2.7 fg/cell/hr PSA with essentially linear kinetics. No effect was seen with dead bacteria, bacterial supernatant or complement upon the PSA production. Live E. coli had no effect for 4–8 hours at which time the cells sloughed and PSA production ceased. No release of stored PSA was seen. These data do not elucidate the reasons for increased serum PSA levels found with acute bacterial prostatitis, but indicate that it is not a storage phenomenon.

AB - Elevations in serum prostate specific antigen (PSA) levels in patients with prostatitis are well known, but the pathophysiologic mechanisms involved with this phenomenon are poorly understood. We have recently evaluated the effect of prostatitis on PSA levels in primates. This data demonstrated a rapid rise in PSA, which subsequently fell along the biological decay curve. This suggested a release of sequestered PSA. We evaluated the effect of infection upon PSA production for the prostatic adenocarcinoma cell line LNCaP. The cell line was determined to produce 9.6 ± 2.7 fg/cell/hr PSA with essentially linear kinetics. No effect was seen with dead bacteria, bacterial supernatant or complement upon the PSA production. Live E. coli had no effect for 4–8 hours at which time the cells sloughed and PSA production ceased. No release of stored PSA was seen. These data do not elucidate the reasons for increased serum PSA levels found with acute bacterial prostatitis, but indicate that it is not a storage phenomenon.

KW - bacterial infection

KW - cell line

UR - http://www.scopus.com/inward/record.url?scp=0026635431&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026635431&partnerID=8YFLogxK

U2 - 10.1002/pros.2990200206

DO - 10.1002/pros.2990200206

M3 - Article

VL - 20

SP - 113

EP - 116

JO - Prostate

JF - Prostate

SN - 0270-4137

IS - 2

ER -