Prostate specific antigen and prostatitis II. PSA production and release kinetics in vitro

Timothy D. Moon, Durwood E. Neal, Sanda Clejan

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

Elevations in serum prostate specific antigen (PSA) levels in patients with prostatitis are well known, but the pathophysiologic mechanisms involved with this phenomenon are poorly understood. We have recently evaluated the effect of prostatitis on PSA levels in primates. This data demonstrated a rapid rise in PSA, which subsequently fell along the biological decay curve. This suggested a release of sequestered PSA. We evaluated the effect of infection upon PSA production for the prostatic adenocarcinoma cell line LNCaP. The cell line was determined to produce 9.6 ± 2.7 fg/cell/hr PSA with essentially linear kinetics. No effect was seen with dead bacteria, bacterial supernatant or complement upon the PSA production. Live E. coli had no effect for 4–8 hours at which time the cells sloughed and PSA production ceased. No release of stored PSA was seen. These data do not elucidate the reasons for increased serum PSA levels found with acute bacterial prostatitis, but indicate that it is not a storage phenomenon.

Original languageEnglish (US)
Pages (from-to)113-116
Number of pages4
JournalThe Prostate
Volume20
Issue number2
DOIs
StatePublished - 1992
Externally publishedYes

Keywords

  • bacterial infection
  • cell line

ASJC Scopus subject areas

  • Oncology
  • Urology

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