Protein engineering to develop a redox insensitive endothelial nitric oxide synthase

Ruslan Rafikov, Sanjiv Kumar, Saurabh Aggarwal, Daniel Pardo, Fabio V. Fonseca, Jessica Ransom, Olga Rafikova, Qiumei Chen, Matthew L. Springer, Stephen M. Black

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

The zinc tetrathiolate (ZnS4) cluster is an important structural feature of endothelial nitric oxide synthase (eNOS). The cluster is located on the dimeric interface and four cysteine residues (C94 and C99 from two adjacent subunits) form a cluster with a Zn ion in the center of a tetrahedral configuration. Due to its high sensitivity to oxidants this cluster is responsible for eNOS dimer destabilization during periods of redox stress. In this work we utilized site directed mutagenesis to replace the redox sensitive cysteine residues in the ZnS4 cluster with redox stable tetra-arginines. Our data indicate that this C94R/C99R eNOS mutant is active. In addition, this mutant protein is insensitive to dimer disruption and inhibition when challenged with hydrogen peroxide (H2O2). Further, the overexpression of the C94R/C99R mutant preserved the angiogenic response in endothelial cells challenged with H2O2. The over-expression of the C94R/C99R mutant preserved the ability of endothelial cells to migrate towards vascular endothelial growth factor (VEGF) and preserved the endothelial monolayer in a scratch wound assay. We propose that this dimer stable eNOS mutant could be utilized in the treatment of diseases in which there is eNOS dysfunction due to high levels of oxidative stress.

Original languageEnglish (US)
Pages (from-to)156-164
Number of pages9
JournalRedox Biology
Volume2
Issue number1
DOIs
Publication statusPublished - Apr 2014

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Keywords

  • Endothelial nitric oxide synthase
  • Protein engineering
  • Redox stability
  • Zinc tetrathiolate cluster

ASJC Scopus subject areas

  • Organic Chemistry
  • Clinical Biochemistry

Cite this

Rafikov, R., Kumar, S., Aggarwal, S., Pardo, D., Fonseca, F. V., Ransom, J., ... Black, S. M. (2014). Protein engineering to develop a redox insensitive endothelial nitric oxide synthase. Redox Biology, 2(1), 156-164. https://doi.org/10.1016/j.redox.2013.12.015