Purified human α-fetoprotein inhibits follicle-stimulating hormone-stimulated estradiol production by porcine granulosa cells in culture

Brooks Allen Keel, Kevan B. Eddy, Yongsheng He, Bhushan K. Gangrade, Jeffrey V. May

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

We have investigated the effects of purified α-fetoprotein (AFP) on follicle-stimulating hormone (FSH)-stimulated estradiol production by porcine granulosa cells in monolayer culture. Granulosa cells isolated from small follicles of prepubertal pigs were cultured for 2 days in 5% fetal bovine serum for attachment and incubated for 3 days in medium containing androstenedione and various treatments. The media were then collected and assayed for estradiol by radioimmunoassay. Human AFP significantly (P < 0.05) and dose-dependently inhibited FSH-stimulated estradiol production with 313 ng/ml AFP returning FSH-stimulated estradiol to basal levels; human serum albumin was without effect. AFP purified from either term cord blood or midtrimester amniotic fluid dose-dependently inhibited estradiol production stimulated by the combination of FSH and insulin-like growth factor-I. Furthermore, 125 ng/ml AFP inhibited estradiol production stimulated by cholera toxin, forskolin and cAMP. In contrast, extracellular accumulation of cAMP and progesterone production was not inhibited by AFP. These data indicate that physiological concentrations of purified AFP significantly and dose-dependently inhibit FSH-stimulated estradiol production by porcine granulosa cells in culture. Since AFP is known to augment growth factor-mediated cell growth, these data suggest that AFP inhibits differentiated functions (such as aromatase) while enhancing the proliferation of porcine granulosa cells.

Original languageEnglish (US)
Pages (from-to)21-25
Number of pages5
JournalMolecular and Cellular Endocrinology
Volume94
Issue number1
DOIs
StatePublished - Jan 1 1993
Externally publishedYes

Fingerprint

Fetal Proteins
Granulosa Cells
Follicle Stimulating Hormone
Estradiol
Swine
Cell Culture Techniques
Aromatase
Androstenedione
Cholera Toxin
Cell growth
Second Pregnancy Trimester
Colforsin
Amniotic Fluid
Fetal Blood
Insulin-Like Growth Factor I
Serum Albumin
Radioimmunoassay
Progesterone
Monolayers
Intercellular Signaling Peptides and Proteins

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Endocrinology

Cite this

Purified human α-fetoprotein inhibits follicle-stimulating hormone-stimulated estradiol production by porcine granulosa cells in culture. / Keel, Brooks Allen; Eddy, Kevan B.; He, Yongsheng; Gangrade, Bhushan K.; May, Jeffrey V.

In: Molecular and Cellular Endocrinology, Vol. 94, No. 1, 01.01.1993, p. 21-25.

Research output: Contribution to journalArticle

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