Pyk2 mediates increased adrenergic contractile responses in arteries from DOCA-salt mice - Vasoactive Peptide Symposium

Fernanda R C Giachini, Fernando S. Carneiro, Victor V. Lima, Zidonia N. Carneiro, Maria Helena C Carvalho, Zuleica B. Fortes, R Clinton Webb, Rita C. Tostes

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The calcium-dependent proline-rich tyrosine kinase (Pyk2), a nonreceptor protein activated by tyrosine phosphorylation, links G protein-coupled receptors to vascular responses. We tested the hypothesis that enhanced vascular reactivity in deoxycorticosterone acetate (DOCA)-salt hypertensive mice is due to increased activation of Pyk2. Aorta and small mesenteric arteries from DOCA-salt and uninephrectomized (UNI) male C57Bl/6 mice were used. Systolic blood pressure (mm Hg) was higher in DOCA (126 ± 3) vs. UNI (100 ± 4) mice. Vascular responses to phenylephrine (1 nM to 100 μM) were greater both in aorta and small mesenteric arteries from DOCA-salt than UNI, but treatment with Tyrphostin A-9 (0.1 μM, Pyk2 inhibitor) abolished the difference among the groups. Pyk2 levels, as well as phospho-Pyk2Tyr402, paxillin, and phospho-paxillinTyr118 were increased in DOCA-salt aorta. Incubation of vessels with Tyrphostin A-9 restored phosphorylation of Pyk2 and paxillin. Increased activation of Pyk2 contributes to increased vascular contractile responses in DOCA-salt mice.

Original languageEnglish (US)
Pages (from-to)431-438
Number of pages8
JournalJournal of the American Society of Hypertension
Volume2
Issue number6
DOIs
StatePublished - Nov 1 2008

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Desoxycorticosterone
Adrenergic Agents
Acetates
Arteries
Salts
Peptides
Blood Vessels
Paxillin
Aorta
Mesenteric Arteries
Phosphorylation
Blood Pressure
Phenylephrine
G-Protein-Coupled Receptors
Proline
Protein-Tyrosine Kinases
Tyrosine
Calcium
Proteins

Keywords

  • Hypertension
  • noradrenaline
  • paxillin
  • vascular smooth muscle cell

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Internal Medicine

Cite this

Giachini, F. R. C., Carneiro, F. S., Lima, V. V., Carneiro, Z. N., Carvalho, M. H. C., Fortes, Z. B., ... Tostes, R. C. (2008). Pyk2 mediates increased adrenergic contractile responses in arteries from DOCA-salt mice - Vasoactive Peptide Symposium. Journal of the American Society of Hypertension, 2(6), 431-438. https://doi.org/10.1016/j.jash.2008.05.001

Pyk2 mediates increased adrenergic contractile responses in arteries from DOCA-salt mice - Vasoactive Peptide Symposium. / Giachini, Fernanda R C; Carneiro, Fernando S.; Lima, Victor V.; Carneiro, Zidonia N.; Carvalho, Maria Helena C; Fortes, Zuleica B.; Webb, R Clinton; Tostes, Rita C.

In: Journal of the American Society of Hypertension, Vol. 2, No. 6, 01.11.2008, p. 431-438.

Research output: Contribution to journalArticle

Giachini, Fernanda R C ; Carneiro, Fernando S. ; Lima, Victor V. ; Carneiro, Zidonia N. ; Carvalho, Maria Helena C ; Fortes, Zuleica B. ; Webb, R Clinton ; Tostes, Rita C. / Pyk2 mediates increased adrenergic contractile responses in arteries from DOCA-salt mice - Vasoactive Peptide Symposium. In: Journal of the American Society of Hypertension. 2008 ; Vol. 2, No. 6. pp. 431-438.
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