Pyk2 mediates increased adrenergic contractile responses in arteries from DOCA-salt mice - Vasoactive Peptide Symposium

Fernanda R.C. Giachini, Fernando S. Carneiro, Victor V. Lima, Zidonia N. Carneiro, Maria Helena C. Carvalho, Zuleica B. Fortes, R. Clinton Webb, Rita C. Tostes

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

The calcium-dependent proline-rich tyrosine kinase (Pyk2), a nonreceptor protein activated by tyrosine phosphorylation, links G protein-coupled receptors to vascular responses. We tested the hypothesis that enhanced vascular reactivity in deoxycorticosterone acetate (DOCA)-salt hypertensive mice is due to increased activation of Pyk2. Aorta and small mesenteric arteries from DOCA-salt and uninephrectomized (UNI) male C57Bl/6 mice were used. Systolic blood pressure (mm Hg) was higher in DOCA (126 ± 3) vs. UNI (100 ± 4) mice. Vascular responses to phenylephrine (1 nM to 100 μM) were greater both in aorta and small mesenteric arteries from DOCA-salt than UNI, but treatment with Tyrphostin A-9 (0.1 μM, Pyk2 inhibitor) abolished the difference among the groups. Pyk2 levels, as well as phospho-Pyk2Tyr402, paxillin, and phospho-paxillinTyr118 were increased in DOCA-salt aorta. Incubation of vessels with Tyrphostin A-9 restored phosphorylation of Pyk2 and paxillin. Increased activation of Pyk2 contributes to increased vascular contractile responses in DOCA-salt mice.

Original languageEnglish (US)
Pages (from-to)431-438
Number of pages8
JournalJournal of the American Society of Hypertension
Volume2
Issue number6
DOIs
StatePublished - Nov 1 2008

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Keywords

  • Hypertension
  • noradrenaline
  • paxillin
  • vascular smooth muscle cell

ASJC Scopus subject areas

  • Internal Medicine
  • Cardiology and Cardiovascular Medicine

Cite this

Giachini, F. R. C., Carneiro, F. S., Lima, V. V., Carneiro, Z. N., Carvalho, M. H. C., Fortes, Z. B., Webb, R. C., & Tostes, R. C. (2008). Pyk2 mediates increased adrenergic contractile responses in arteries from DOCA-salt mice - Vasoactive Peptide Symposium. Journal of the American Society of Hypertension, 2(6), 431-438. https://doi.org/10.1016/j.jash.2008.05.001