Quantal analysis suggests strong involvement of presynaptic mechanisms during the initial 3 h maintenance of long-term potentiation in rat hippocampal CA1 area in vitro

M. V. Sokolov, A. V. Rossokhin, A. V. Astrelin, Julietta Uta Frey, L. L. Voronin

Research output: Contribution to journalArticle

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Abstract

Long-term potentiation (LTP) is the most prominent model to study neuronal plasticity. Previous studies using quantal analysis of an early stage of LTP in the CA1 hippocampal region (<1 h after induction) suggested increases in both the mean number of transmitter quanta released by each presynaptic pulse (m, quantal content) and postsynaptic effect of a single quantum (v, quantal size). When LTP was large, it was m that increased predominantly suggesting prevailing presynaptic contribution. However, LTP consists of several temporary phases with presumably different mechanisms. Here we recorded excitatory postsynaptic potentials from CA1 hippocampal slices before and up to 3.5 h after LTP induction. A new version of the noise deconvolution revealed significant increases in m with smaller and often not statistically significant changes in v. The changes in m were similar for both early (<1 h) and later (1-3 h) post-tetanic periods and correlated with LTP magnitude. The coefficient of variation of the response amplitude and the number of failures decreased during both early and late post-tetanic periods. The results suggest that both early (<0.5 h) and later LTP components (0.5-3 h) are maintained by presynaptic changes, which include increases in release probabilities and the number of effective release sites. In addition initially silent synapses can be converted into effective ones due to either pre- or postsynaptic rearrangements. If this occurs, our data indicate that the number and the efficacy of the receptors in the new transmission sites are approximately similar to those in the previously effective sites.

Original languageEnglish (US)
Pages (from-to)61-75
Number of pages15
JournalBrain Research
Volume957
Issue number1
DOIs
StatePublished - Dec 6 2002

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Long-Term Potentiation
Hippocampal CA1 Region
Neuronal Plasticity
Excitatory Postsynaptic Potentials
In Vitro Techniques
Synapses
Noise

Keywords

  • Quantal analysis
  • Rat

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

Cite this

Quantal analysis suggests strong involvement of presynaptic mechanisms during the initial 3 h maintenance of long-term potentiation in rat hippocampal CA1 area in vitro. / Sokolov, M. V.; Rossokhin, A. V.; Astrelin, A. V.; Frey, Julietta Uta; Voronin, L. L.

In: Brain Research, Vol. 957, No. 1, 06.12.2002, p. 61-75.

Research output: Contribution to journalArticle

Sokolov, M. V. ; Rossokhin, A. V. ; Astrelin, A. V. ; Frey, Julietta Uta ; Voronin, L. L. / Quantal analysis suggests strong involvement of presynaptic mechanisms during the initial 3 h maintenance of long-term potentiation in rat hippocampal CA1 area in vitro. In: Brain Research. 2002 ; Vol. 957, No. 1. pp. 61-75.
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