Quantities of adult, fetal and embryonic globin chains in the blood of eighteen- to twenty-week-old human fetuses

Ferdane Kutlar, Hugo Moscoso, Charles R. Kiefer, Frederick A. Garver, Sinan Beksaç, Lütfi Önderoglu, Aytemiz Gurgey, Çigdem Altay, Titus H J Huisman

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The prenatal diagnostic program, established at Hacettepe University in Ankara for the purpose of detecting β-thalassemia (β-thal), sickle cell anemia (SS), and Hb S-β-thal, offered the opportunity of evaluating the relative quantities of adult (βA, βS), fetal (Gγ, Aγ, AγT), and embryonic (ε, ζ) chains in 26 fetuses, aged 18-20 weeks. Methodology involved micro high-performance liquid chromatographic (HPLC) procedures and immunology using an mAb, specific for the embryonic ε chain. A good correlation was observed between the β γ in vitro chain synthesis ratio and the level of βA and/or βS chains determined by reversed-phase HPLC; the combination of these two sets of data strengthens the prenatal diagnostic approach of detecting β-thal major but not β-thal trait. The levels of the different γ chains were about as observed in newborn babies; the frequency of the AγT variant in the 26 fetuses was the same as observed for a larger group of Turkisch newborn babies. The level of the embryonic ζ chain was higher than seen in full-term babies and varied between 0 and 1.3%; 5 of the 26 fetuses showed the complete absence of ζ. The embryonic ε chain was not detectable, not even in babies with β-thal major. These data indicate that the synthesis of ε is completely turned off in fetuses at the age of 18-20 weeks, while that of ζ continues, albeit at a low level.

Original languageEnglish (US)
Pages (from-to)359-368
Number of pages10
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Volume567
Issue number2
DOIs
StatePublished - Jul 5 1991

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Globins
Fetus
Blood
Program diagnostics
Immunology
Liquids
Thalassemia
Sickle Cell Anemia
Allergy and Immunology

ASJC Scopus subject areas

  • Chemistry(all)
  • Clinical Biochemistry
  • Molecular Medicine

Cite this

Quantities of adult, fetal and embryonic globin chains in the blood of eighteen- to twenty-week-old human fetuses. / Kutlar, Ferdane; Moscoso, Hugo; Kiefer, Charles R.; Garver, Frederick A.; Beksaç, Sinan; Önderoglu, Lütfi; Gurgey, Aytemiz; Altay, Çigdem; Huisman, Titus H J.

In: Journal of Chromatography B: Biomedical Sciences and Applications, Vol. 567, No. 2, 05.07.1991, p. 359-368.

Research output: Contribution to journalArticle

Kutlar, F, Moscoso, H, Kiefer, CR, Garver, FA, Beksaç, S, Önderoglu, L, Gurgey, A, Altay, Ç & Huisman, THJ 1991, 'Quantities of adult, fetal and embryonic globin chains in the blood of eighteen- to twenty-week-old human fetuses', Journal of Chromatography B: Biomedical Sciences and Applications, vol. 567, no. 2, pp. 359-368. https://doi.org/10.1016/0378-4347(91)80142-Y
Kutlar, Ferdane ; Moscoso, Hugo ; Kiefer, Charles R. ; Garver, Frederick A. ; Beksaç, Sinan ; Önderoglu, Lütfi ; Gurgey, Aytemiz ; Altay, Çigdem ; Huisman, Titus H J. / Quantities of adult, fetal and embryonic globin chains in the blood of eighteen- to twenty-week-old human fetuses. In: Journal of Chromatography B: Biomedical Sciences and Applications. 1991 ; Vol. 567, No. 2. pp. 359-368.
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AU - Beksaç, Sinan

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