TY - JOUR
T1 - Quasispecies development of Helicobacter pylori observed in paired isolates obtained years apart from the same host
AU - Kuipers, Ernst J.
AU - Israel, Dawn A.
AU - Kusters, Johannes G.
AU - Gerrits, Monique M.
AU - Weel, Jan
AU - Van Der Ende, Arie
AU - Van der Hulst, René W.M.
AU - Wirth, Hans P.
AU - Höök-Nikanne, Johanna
AU - Thompson, Stuart A.
AU - Blaser, Martin J.
N1 - Funding Information:
Financial support: Stichting van Helten of the Royal Dutch Academy of Sciences; NIH (DK-53707); the Medical Research Service of the Department of Veterans Affairs; and the Iris and Homer Akers Fellowship in Infectious Disease.
PY - 2000
Y1 - 2000
N2 - Helicobacter pylori isolates show greater genetic diversity than other bacterial species studied, but the basis for this phenomenon is unknown. Whether detectable genomic mutation appears within an H. pylori population during persistent colonization was investigated. Paired H. pylori populations obtained across 7- to 10-year intervals from 13 patients were characterized by use of methods including polymerase chain reaction (PCR) genotyping for cagA, vacA, iceA, recA, and IS605; random arbitrarily primed DNA (RAPD)-PCR and amplified fragment length polymorphism (AFLP) analysis; and ELISA, to determine Lewis phenotypes. Genotyping, including recA sequence analysis, revealed that initial and follow-up populations represented the same population in 11 patients (85%). Nevertheless, distinct dissimilarities were shown within each of these 11 pairs by both RAPD-PCR and AFLP analyses. During follow-up, Lewis-y levels, but not Lewis-x levels, decreased significantly. The changes detected by RAPD-PCR and AFLP indicate that genetic drift occurs within H. pylori populations over the course of years of colonization of a single host.
AB - Helicobacter pylori isolates show greater genetic diversity than other bacterial species studied, but the basis for this phenomenon is unknown. Whether detectable genomic mutation appears within an H. pylori population during persistent colonization was investigated. Paired H. pylori populations obtained across 7- to 10-year intervals from 13 patients were characterized by use of methods including polymerase chain reaction (PCR) genotyping for cagA, vacA, iceA, recA, and IS605; random arbitrarily primed DNA (RAPD)-PCR and amplified fragment length polymorphism (AFLP) analysis; and ELISA, to determine Lewis phenotypes. Genotyping, including recA sequence analysis, revealed that initial and follow-up populations represented the same population in 11 patients (85%). Nevertheless, distinct dissimilarities were shown within each of these 11 pairs by both RAPD-PCR and AFLP analyses. During follow-up, Lewis-y levels, but not Lewis-x levels, decreased significantly. The changes detected by RAPD-PCR and AFLP indicate that genetic drift occurs within H. pylori populations over the course of years of colonization of a single host.
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U2 - 10.1086/315173
DO - 10.1086/315173
M3 - Article
C2 - 10608776
AN - SCOPUS:0033983312
SN - 0022-1899
VL - 181
SP - 273
EP - 282
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
IS - 1
ER -
