Quasispecies development of Helicobacter pylori observed in paired isolates obtained years apart from the same host

Ernst J. Kuipers, Dawn A. Israel, Johannes G. Kusters, Monique M. Gerrits, Jan Weel, Arie Van Der Ende, René W.M. Van der Hulst, Hans P. Wirth, Johanna Höök-Nikanne, Stuart A. Thompson, Martin J. Blaser

Research output: Contribution to journalArticle

141 Scopus citations

Abstract

Helicobacter pylori isolates show greater genetic diversity than other bacterial species studied, but the basis for this phenomenon is unknown. Whether detectable genomic mutation appears within an H. pylori population during persistent colonization was investigated. Paired H. pylori populations obtained across 7- to 10-year intervals from 13 patients were characterized by use of methods including polymerase chain reaction (PCR) genotyping for cagA, vacA, iceA, recA, and IS605; random arbitrarily primed DNA (RAPD)-PCR and amplified fragment length polymorphism (AFLP) analysis; and ELISA, to determine Lewis phenotypes. Genotyping, including recA sequence analysis, revealed that initial and follow-up populations represented the same population in 11 patients (85%). Nevertheless, distinct dissimilarities were shown within each of these 11 pairs by both RAPD-PCR and AFLP analyses. During follow-up, Lewis-y levels, but not Lewis-x levels, decreased significantly. The changes detected by RAPD-PCR and AFLP indicate that genetic drift occurs within H. pylori populations over the course of years of colonization of a single host.

Original languageEnglish (US)
Pages (from-to)273-282
Number of pages10
JournalJournal of Infectious Diseases
Volume181
Issue number1
DOIs
StatePublished - 2000

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

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    Kuipers, E. J., Israel, D. A., Kusters, J. G., Gerrits, M. M., Weel, J., Van Der Ende, A., Van der Hulst, R. W. M., Wirth, H. P., Höök-Nikanne, J., Thompson, S. A., & Blaser, M. J. (2000). Quasispecies development of Helicobacter pylori observed in paired isolates obtained years apart from the same host. Journal of Infectious Diseases, 181(1), 273-282. https://doi.org/10.1086/315173