Rab26 modulates the cell surface transport of α2- adrenergic receptors from the Golgi

Chunman Li, Yi Fan, Tien Hung Lan, Nevin A. Lambert, Guangyu Wu

Research output: Contribution to journalArticle

24 Scopus citations

Abstract

The molecular mechanisms underlying the transport from the Golgi to the cell surface of G protein-coupled receptors remain poorly elucidated. Here we determined the role of Rab26, a Ras-like small GTPase involved in vesicle-mediated secretion, in the cell surface export of α2- adrenergic receptors. We found that transient expression of Rab26 mutants and siRNA-mediated depletion of Rab26 significantly attenuated the cell surface numbers of α2A-AR and α2B-AR, as well as ERK1/2 activation by α2B-AR. Furthermore, the receptors were extensively arrested in the Golgi by Rab26 mutants and siRNA. Moreover, Rab26 directly and activation-dependently interacted with α2B-AR, specifically the third intracellular loop. These data demonstrate that the small GTPase Rab26 regulates the Golgi to cell surface traffic of α2-adrenergic receptors, likely through a physical interaction. These data also provide the first evidence implicating an important function of Rab26 in coordinating plasma membrane protein transport.

Original languageEnglish (US)
Pages (from-to)42784-42794
Number of pages11
JournalJournal of Biological Chemistry
Volume287
Issue number51
DOIs
StatePublished - Dec 14 2012

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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