Radiation-induced meningioma

A distinct molecular genetic pattern?

Yigal Shoshan, Olga Chernova, Sin Soo Jeun, Robert P. Somerville, Zvi Israel, Gene H. Barnett, John Kenneth Cowell

Research output: Contribution to journalReview article

73 Citations (Scopus)

Abstract

Radiation-induced meningiomas arise after low-dose irradiation treatment of certain medical conditions and are recognized as clinically separate from sporadic meningioma. These tumors are often aggressive or malignant, they are likely to be multiple, and they have a high recurrence rate following treatment compared with sporadic meningiomas. To understand the molecular mechanism by which radiation-induced meningioma (RIM) arise, we compared genetic changes in 7 RIM and 8 sporadic meningioma (SM) samples. The presence of mutations in the 17 exons of the neurofibromatosis type 2 (NF2) gene, which has been shown to be inactivated in sporadic meningiomas, was analyzed in RIM and SM using single-strand conformation polymorphism (SSCP) and DNA sequencing. In contrast to SM, which showed NF2 mutations in 50% of specimens, no mutations were found in RIM. In addition, Western blot analysis of schwannomin/merlin protein, the NF2 gene product, demonstrated protein levels comparable to normal brain in 4/4 RIM tumor samples analyzed. Loss of heterozygosity (LOH) of genomic regions, which were reported for SM, was also analyzed in all cases of RIM using 22 polymorphic DNA markers. Allele losses were found on chromosomes 1p (4/7), 9p (2/7), 19q (2/7), 22q (2/7), and 18q (1/7). From these observations we conclude that unlike sporadic meningiomas, NF2 gene inactivation and chromosome 22q deletions are far less frequent in RIM, and their role in meningioma development following low dose irradiation is less significant. Other chromosomal lesions, especially loss of 1p, possibly induced by irradiation, may be more important in the development of these tumors.

Original languageEnglish (US)
Pages (from-to)614-620
Number of pages7
JournalJournal of Neuropathology and Experimental Neurology
Volume59
Issue number7
DOIs
StatePublished - Jan 1 2000
Externally publishedYes

Fingerprint

Meningioma
Molecular Biology
Neurofibromatosis 2
Neurofibromin 2
Neurofibromatosis 2 Genes
Mutation
Radiation induced meningioma
Neoplasms
Chromosome Deletion
Chromosomes, Human, Pair 4
Loss of Heterozygosity
Gene Silencing
DNA Sequence Analysis
Genetic Markers
Exons
Western Blotting
Alleles
Recurrence
Brain

Keywords

  • Loss of heterozygosity
  • Meningioma
  • NF2 mutations
  • Radiation
  • Tumor suppressor genes

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Neurology
  • Clinical Neurology
  • Cellular and Molecular Neuroscience

Cite this

Radiation-induced meningioma : A distinct molecular genetic pattern? / Shoshan, Yigal; Chernova, Olga; Jeun, Sin Soo; Somerville, Robert P.; Israel, Zvi; Barnett, Gene H.; Cowell, John Kenneth.

In: Journal of Neuropathology and Experimental Neurology, Vol. 59, No. 7, 01.01.2000, p. 614-620.

Research output: Contribution to journalReview article

Shoshan, Y, Chernova, O, Jeun, SS, Somerville, RP, Israel, Z, Barnett, GH & Cowell, JK 2000, 'Radiation-induced meningioma: A distinct molecular genetic pattern?', Journal of Neuropathology and Experimental Neurology, vol. 59, no. 7, pp. 614-620. https://doi.org/10.1093/jnen/59.7.614
Shoshan Y, Chernova O, Jeun SS, Somerville RP, Israel Z, Barnett GH et al. Radiation-induced meningioma: A distinct molecular genetic pattern? Journal of Neuropathology and Experimental Neurology. 2000 Jan 1;59(7):614-620. https://doi.org/10.1093/jnen/59.7.614
Shoshan, Yigal ; Chernova, Olga ; Jeun, Sin Soo ; Somerville, Robert P. ; Israel, Zvi ; Barnett, Gene H. ; Cowell, John Kenneth. / Radiation-induced meningioma : A distinct molecular genetic pattern?. In: Journal of Neuropathology and Experimental Neurology. 2000 ; Vol. 59, No. 7. pp. 614-620.
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