Regional localisation of tri- and tetranucleotide repeat sequence-containing cosmids on chromosome 13

Lesleyann Hawthorn, B. Kapanadse, N. Yankovsky, John Kenneth Cowell

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A human chromosome 13-specific cosmid library has been screened with oligonucleotides containing tri- and tetranucleotide repeats with motifs: GACA, GACT, CAC, TCC. 20 cosmid clones were identified and their physical location on chromosome 13 was determined using fluorescence in situ hybridisation. Over 80% of the cosmids were detected using tetranucleotide repeats of which 50% were GACT, but hese were distributed along the length of the chromosome. Clones from the 13q12→q14 and 13q32→q34 regions were apparently overrepresented and 10% of cosmids were localised to the short arm of the chromosome. These simple tandem repeats will be an important addition to the genetic mapping strategy for human chromosome 13.

Original languageEnglish (US)
Pages (from-to)102-103
Number of pages2
JournalCytogenetic and Genome Research
Volume70
Issue number1-2
DOIs
StatePublished - Jan 1 1995
Externally publishedYes

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Trinucleotide Repeats
Chromosomes, Human, Pair 13
Cosmids
Microsatellite Repeats
Human Chromosomes
Clone Cells
Chromosomes
Tandem Repeat Sequences
Fluorescence In Situ Hybridization
Oligonucleotides

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Genetics(clinical)

Cite this

Regional localisation of tri- and tetranucleotide repeat sequence-containing cosmids on chromosome 13. / Hawthorn, Lesleyann; Kapanadse, B.; Yankovsky, N.; Cowell, John Kenneth.

In: Cytogenetic and Genome Research, Vol. 70, No. 1-2, 01.01.1995, p. 102-103.

Research output: Contribution to journalArticle

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