Regulation of myosin activation during cell-cell contact formation by Par3-Lgl antagonism

Entosis without matrix detachment

Qingwen Wan, Jing Liu, Zhen Zheng, Huabin Zhu, Xiaogang Chu, Zheng Dong, Shuang Huang, Quansheng Du

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Cell-cell contact formation following cadherin engagement requires actomyosin contraction along the periphery of cell-cell contact. The molecular mechanisms that regulate myosin activation during this process are not clear. In this paper, we show that two polarity proteins, partitioning defective 3 homologue (Par3) and mammalian homologues of Drosophila Lethal (2) Giant Larvae (Lgl1/2), antagonize each other in modulating myosin II activation during cell-cell contact formation in Madin-Darby canine kidney cells. While overexpression of Lgl1/2 or depletion of endogenous Par3 leads to enhanced myosin II activation, knockdown of Lgl1/2 does the opposite. Intriguingly, altering the counteraction between Par3 and Lgl1/2 induces cell-cell internalization during early cell-cell contact formation, which involves active invasion of the lateral cell-cell contact underneath the apical-junctional complexes and requires activation of the Rho-Rho-associated, coiled-coil containing protein kinase (ROCK)-myosin pathway. This is followed by predominantly nonapoptotic cell-in-cell death of the internalized cells and frequent aneuploidy of the host cells. Such effects are reminiscent of entosis, a recently described process observed when mammary gland epithelial cells were cultured in suspension. We propose that entosis could occur without matrix detachment and that overactivation of myosin or unbalanced myosin activation between contacting cells may be the driving force for entosis in epithelial cells.

Original languageEnglish (US)
Pages (from-to)2076-2091
Number of pages16
JournalMolecular Biology of the Cell
Volume23
Issue number11
DOIs
StatePublished - Jun 1 2012

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Entosis
Myosins
Myosin Type II
Cell-in-Cell Formation
Epithelial Cells

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Regulation of myosin activation during cell-cell contact formation by Par3-Lgl antagonism : Entosis without matrix detachment. / Wan, Qingwen; Liu, Jing; Zheng, Zhen; Zhu, Huabin; Chu, Xiaogang; Dong, Zheng; Huang, Shuang; Du, Quansheng.

In: Molecular Biology of the Cell, Vol. 23, No. 11, 01.06.2012, p. 2076-2091.

Research output: Contribution to journalArticle

Wan, Qingwen ; Liu, Jing ; Zheng, Zhen ; Zhu, Huabin ; Chu, Xiaogang ; Dong, Zheng ; Huang, Shuang ; Du, Quansheng. / Regulation of myosin activation during cell-cell contact formation by Par3-Lgl antagonism : Entosis without matrix detachment. In: Molecular Biology of the Cell. 2012 ; Vol. 23, No. 11. pp. 2076-2091.
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