Regulation of protein kinase C activity by gangliosides.

D. Kreutter, J. Y. Kim, J. R. Goldenring, H. Rasmussen, C. Ukomadu, R. J. DeLorenzo, Robert K Yu

Research output: Contribution to journalArticle

203 Citations (Scopus)

Abstract

The activity of protein kinase C (Ca2+/phospholipid-dependent enzyme) in the presence of phosphatidylserine and its physiological regulator, diacylglycerol, could be suppressed by a mixture of brain gangliosides. Half-maximal inhibition was observed at 30 microM and was nearly complete at 100 microM. Inhibition was observed at all concentrations of Ca2+ between 10(-8) and 10(-4) M. Inhibition of protein kinase C activity could not be reversed by increasing the concentration of diacylglycerol or the substrate, histone. Inhibition was also observed when myelin basic protein or a synthetic myelin basic protein peptide was used as substrate. Among the individual gangliosides, the rank order of potency was GT1b greater than GD1a = GD1b greater than GM3 = GM1. Our results suggest that gangliosides may regulate the responsiveness of protein kinase C to diacylglycerol.

Original languageEnglish (US)
Pages (from-to)1633-1637
Number of pages5
JournalJournal of Biological Chemistry
Volume262
Issue number4
StatePublished - Feb 5 1987
Externally publishedYes

Fingerprint

Gangliosides
Diglycerides
Protein Kinase C
Myelin Basic Protein
Phosphatidylserines
Substrates
Histones
Brain
Phospholipids
Peptides
Enzymes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Kreutter, D., Kim, J. Y., Goldenring, J. R., Rasmussen, H., Ukomadu, C., DeLorenzo, R. J., & Yu, R. K. (1987). Regulation of protein kinase C activity by gangliosides. Journal of Biological Chemistry, 262(4), 1633-1637.

Regulation of protein kinase C activity by gangliosides. / Kreutter, D.; Kim, J. Y.; Goldenring, J. R.; Rasmussen, H.; Ukomadu, C.; DeLorenzo, R. J.; Yu, Robert K.

In: Journal of Biological Chemistry, Vol. 262, No. 4, 05.02.1987, p. 1633-1637.

Research output: Contribution to journalArticle

Kreutter, D, Kim, JY, Goldenring, JR, Rasmussen, H, Ukomadu, C, DeLorenzo, RJ & Yu, RK 1987, 'Regulation of protein kinase C activity by gangliosides.', Journal of Biological Chemistry, vol. 262, no. 4, pp. 1633-1637.
Kreutter D, Kim JY, Goldenring JR, Rasmussen H, Ukomadu C, DeLorenzo RJ et al. Regulation of protein kinase C activity by gangliosides. Journal of Biological Chemistry. 1987 Feb 5;262(4):1633-1637.
Kreutter, D. ; Kim, J. Y. ; Goldenring, J. R. ; Rasmussen, H. ; Ukomadu, C. ; DeLorenzo, R. J. ; Yu, Robert K. / Regulation of protein kinase C activity by gangliosides. In: Journal of Biological Chemistry. 1987 ; Vol. 262, No. 4. pp. 1633-1637.
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