Regulation of transforming growth factor-beta, type III collagen, and fibronectin by dichloroacetic acid in human fibroblasts from normal peritoneum and adhesions

Michael Peter Diamond, Eslam El-Hammady, Rona Wang, Ghassan Saed

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Objective: To examine the role of aerobic metabolism in fibroblasts from normal peritoneum and adhesions in the differential expression of extracellular matrix (ECM) and transforming growth factor-beta (TGF-β), an inflammatory cytokine that regulates ECM expression. Design: Cell culture under normoxic and hypoxic conditions. Setting: University research laboratory. Patient(s): Human fibroblasts cultures from normal peritoneum and adhesions. Intervention(s): Exposure to dichloroacetic acid (DCA), which activates pyruvate dehydrogenase, for 24 hours under normal and hypoxic (2% O2) conditions. Main Outcome Measure(s): Multiplex reverse transcriptase polymerase chain reaction (RT/PCR) of type III collagen, fibronectin, TGF-β1, and β-actin was performed, with analysis of PCR-amplified products performed by densimetric analysis of gel bands using the National Institutes of Health Image analysis program. Result(s): DCA inhibited human peritoneal fibroblast and adhesion fibroblast TGF-β1 mRNA expression under normoxic and hypoxic conditions. DCA also markedly reduced fibronectin and type III collagen expression under hypoxic conditions in fibroblasts from normal peritoneum and adhesions. Conclusion(s): These observations provide further support for the suggestion that regulation of metabolic activity of peritoneal cells may provide a target for interventions designed to reduce postoperative adhesions.

Original languageEnglish (US)
Pages (from-to)1161-1167
Number of pages7
JournalFertility and Sterility
Volume79
Issue number5
DOIs
StatePublished - May 1 2003
Externally publishedYes

Fingerprint

Dichloroacetic Acid
Collagen Type III
Peritoneum
Fibronectins
Transforming Growth Factor beta
Fibroblasts
Extracellular Matrix
Fibroblast Growth Factors
Multiplex Polymerase Chain Reaction
National Institutes of Health (U.S.)
Reverse Transcriptase Polymerase Chain Reaction
Pyruvic Acid
Actins
Oxidoreductases
Cell Culture Techniques
Gels
Outcome Assessment (Health Care)
Cytokines
Polymerase Chain Reaction
Messenger RNA

Keywords

  • Adhesions
  • Dichloroacetic acid
  • Fibronectin
  • Hypoxia
  • Postoperative adhesions
  • Transforming growth factor beta
  • Type III collagen

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynecology

Cite this

Regulation of transforming growth factor-beta, type III collagen, and fibronectin by dichloroacetic acid in human fibroblasts from normal peritoneum and adhesions. / Diamond, Michael Peter; El-Hammady, Eslam; Wang, Rona; Saed, Ghassan.

In: Fertility and Sterility, Vol. 79, No. 5, 01.05.2003, p. 1161-1167.

Research output: Contribution to journalArticle

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