Relationship between cytokine levels in serum and gingival crevicular fluid (GCF) in pregnant women

Tiago Fiorini, Priscila Vianna, Patricia Weidlich, Marta Liliana Musskopf, Carlos Heitor Cunha Moreira, José Artur Bogo Chies, Cassiano Kuchenbecker Rösing, Rui Vicente Oppermann, Cristiano Susin

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Background: Periodontal disease has been linked to systemic diseases/disorders and a low-grade systemic inflammatory status originated from periodontitis has been proposed as a possible explanation for this association. This study evaluates the relationship, early in pregnancy, between gingival crevicular fluid (GCF) and serum levels of a panel of cytokines that have been implicated in PTB and periodontal disease. Methods: One hundred pregnant women aged 18-35. years old with a gestational age up to 20. weeks were included (mean ± SD gestational age:16.1 ± 3.5. weeks). Four periodontal sites per subject were randomly selected for GCF collection. Serum and GCF levels of IL-1β, IL-6, IL-8, IL-10, IL-12p70 and TNF-α were analyzed using a cytometric bead array. Regression and correlation analyses were used to assess the relationship between serum and GCF cytokine levels. Results: Participants had widespread periodontal inflammation but limited periodontal destruction. Cytokine levels were significantly higher in GCF than serum for all cytokines but IL-10. GCF levels had small but significant effect on serum levels for IL-10 (β= 0.34 ± 0.09, p< 0.01), IL-12p70 (β= 0.48 ± 0.08, p< 0.01) and TNF-α (β= 0.29 ± 0.09, p< 0.01). Periodontal probing depth and bleeding on probing were significantly associated with GCF levels for IL-1β, IL-6 and IL-8; however, they had negligible effect on serum cytokine levels. Correlation between GCF and serum levels was non-significant, except for IL-12p70, which showed a significant but small correlation between the two sources (r= 0.32, p= 0.001). Conclusions: GCF cytokine levels were not strongly associated with serum cytokine levels in pregnant women with widespread periodontal inflammation but limited periodontal destruction.

Original languageEnglish (US)
Pages (from-to)34-39
Number of pages6
JournalCytokine
Volume58
Issue number1
DOIs
StatePublished - Apr 1 2012

Fingerprint

Gingival Crevicular Fluid
Pregnant Women
Cytokines
Fluids
Serum
Interleukin-10
Periodontal Diseases
Interleukin-8
Interleukin-1
Gestational Age
Interleukin-6
Inflammation
Periodontitis
Regression Analysis
Hemorrhage
Pregnancy

Keywords

  • Cross-sectional study
  • Gingival crevicular fluid
  • Interleukins
  • Low-grade inflammation
  • Periodontal disease

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Biochemistry
  • Hematology
  • Molecular Biology

Cite this

Fiorini, T., Vianna, P., Weidlich, P., Musskopf, M. L., Moreira, C. H. C., Chies, J. A. B., ... Susin, C. (2012). Relationship between cytokine levels in serum and gingival crevicular fluid (GCF) in pregnant women. Cytokine, 58(1), 34-39. https://doi.org/10.1016/j.cyto.2011.12.015

Relationship between cytokine levels in serum and gingival crevicular fluid (GCF) in pregnant women. / Fiorini, Tiago; Vianna, Priscila; Weidlich, Patricia; Musskopf, Marta Liliana; Moreira, Carlos Heitor Cunha; Chies, José Artur Bogo; Rösing, Cassiano Kuchenbecker; Oppermann, Rui Vicente; Susin, Cristiano.

In: Cytokine, Vol. 58, No. 1, 01.04.2012, p. 34-39.

Research output: Contribution to journalArticle

Fiorini, T, Vianna, P, Weidlich, P, Musskopf, ML, Moreira, CHC, Chies, JAB, Rösing, CK, Oppermann, RV & Susin, C 2012, 'Relationship between cytokine levels in serum and gingival crevicular fluid (GCF) in pregnant women', Cytokine, vol. 58, no. 1, pp. 34-39. https://doi.org/10.1016/j.cyto.2011.12.015
Fiorini T, Vianna P, Weidlich P, Musskopf ML, Moreira CHC, Chies JAB et al. Relationship between cytokine levels in serum and gingival crevicular fluid (GCF) in pregnant women. Cytokine. 2012 Apr 1;58(1):34-39. https://doi.org/10.1016/j.cyto.2011.12.015
Fiorini, Tiago ; Vianna, Priscila ; Weidlich, Patricia ; Musskopf, Marta Liliana ; Moreira, Carlos Heitor Cunha ; Chies, José Artur Bogo ; Rösing, Cassiano Kuchenbecker ; Oppermann, Rui Vicente ; Susin, Cristiano. / Relationship between cytokine levels in serum and gingival crevicular fluid (GCF) in pregnant women. In: Cytokine. 2012 ; Vol. 58, No. 1. pp. 34-39.
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abstract = "Background: Periodontal disease has been linked to systemic diseases/disorders and a low-grade systemic inflammatory status originated from periodontitis has been proposed as a possible explanation for this association. This study evaluates the relationship, early in pregnancy, between gingival crevicular fluid (GCF) and serum levels of a panel of cytokines that have been implicated in PTB and periodontal disease. Methods: One hundred pregnant women aged 18-35. years old with a gestational age up to 20. weeks were included (mean ± SD gestational age:16.1 ± 3.5. weeks). Four periodontal sites per subject were randomly selected for GCF collection. Serum and GCF levels of IL-1β, IL-6, IL-8, IL-10, IL-12p70 and TNF-α were analyzed using a cytometric bead array. Regression and correlation analyses were used to assess the relationship between serum and GCF cytokine levels. Results: Participants had widespread periodontal inflammation but limited periodontal destruction. Cytokine levels were significantly higher in GCF than serum for all cytokines but IL-10. GCF levels had small but significant effect on serum levels for IL-10 (β= 0.34 ± 0.09, p< 0.01), IL-12p70 (β= 0.48 ± 0.08, p< 0.01) and TNF-α (β= 0.29 ± 0.09, p< 0.01). Periodontal probing depth and bleeding on probing were significantly associated with GCF levels for IL-1β, IL-6 and IL-8; however, they had negligible effect on serum cytokine levels. Correlation between GCF and serum levels was non-significant, except for IL-12p70, which showed a significant but small correlation between the two sources (r= 0.32, p= 0.001). Conclusions: GCF cytokine levels were not strongly associated with serum cytokine levels in pregnant women with widespread periodontal inflammation but limited periodontal destruction.",
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AU - Vianna, Priscila

AU - Weidlich, Patricia

AU - Musskopf, Marta Liliana

AU - Moreira, Carlos Heitor Cunha

AU - Chies, José Artur Bogo

AU - Rösing, Cassiano Kuchenbecker

AU - Oppermann, Rui Vicente

AU - Susin, Cristiano

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N2 - Background: Periodontal disease has been linked to systemic diseases/disorders and a low-grade systemic inflammatory status originated from periodontitis has been proposed as a possible explanation for this association. This study evaluates the relationship, early in pregnancy, between gingival crevicular fluid (GCF) and serum levels of a panel of cytokines that have been implicated in PTB and periodontal disease. Methods: One hundred pregnant women aged 18-35. years old with a gestational age up to 20. weeks were included (mean ± SD gestational age:16.1 ± 3.5. weeks). Four periodontal sites per subject were randomly selected for GCF collection. Serum and GCF levels of IL-1β, IL-6, IL-8, IL-10, IL-12p70 and TNF-α were analyzed using a cytometric bead array. Regression and correlation analyses were used to assess the relationship between serum and GCF cytokine levels. Results: Participants had widespread periodontal inflammation but limited periodontal destruction. Cytokine levels were significantly higher in GCF than serum for all cytokines but IL-10. GCF levels had small but significant effect on serum levels for IL-10 (β= 0.34 ± 0.09, p< 0.01), IL-12p70 (β= 0.48 ± 0.08, p< 0.01) and TNF-α (β= 0.29 ± 0.09, p< 0.01). Periodontal probing depth and bleeding on probing were significantly associated with GCF levels for IL-1β, IL-6 and IL-8; however, they had negligible effect on serum cytokine levels. Correlation between GCF and serum levels was non-significant, except for IL-12p70, which showed a significant but small correlation between the two sources (r= 0.32, p= 0.001). Conclusions: GCF cytokine levels were not strongly associated with serum cytokine levels in pregnant women with widespread periodontal inflammation but limited periodontal destruction.

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KW - Interleukins

KW - Low-grade inflammation

KW - Periodontal disease

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