Relative importance of inositol (1,4,5)trisphosphate and inositol (1,3,4,5)tetrakisphosphate in Entamoeba histolytica

Banabihari Giri, Sanghamitra Raha, Bhudeb Bhattacharyya, Susweta Biswas, Birendra B. Biswas

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

[3H]Inositol tetrakisphosphate (Ins(1,3,4,5)P4) binding sites which were poorly displaced by unlabelled inositol (1,4,5)-trisphosphate (Ins(1,4,5)P3) were detected in membrane fractions of Entamoeba histolytica. Similarly, unlabelled Ins(1,3,4,5)P4 was 30-fold less efficient in displacing [3H]Ins(1,4,5)P3 binding. pH sensitivities of binding of the two isomers were markedly different. Scatchard analysis of the data revealed single binding sites and similar receptor densities for each of the two isomers. Formation of both Ins(1,4,5)P3 and Ins(1,3,4,5)P4 in E. histolytica was also demonstrated. Calcium release studies showed that after treatment with a saturating dose of either Ins(1,4,5)P3 or Ins(1,3,4,5)P4 the other inositol polyphosphate could partially revive the response to a subsequent addition of the first inducer. Our data clearly demonstrate that Ins(1,4,5)P3 and Ins(1,3,4,5)P4 are two equally important but independent second messengers in E. histolytica.

Original languageEnglish (US)
Pages (from-to)109-112
Number of pages4
JournalFEBS Letters
Volume393
Issue number1
DOIs
StatePublished - Sep 9 1996

Fingerprint

Entamoeba histolytica
Inositol 1,4,5-Trisphosphate
Inositol
Isomers
Binding Sites
Polyphosphates
Second Messenger Systems
inositol-1,3,4,5-tetrakisphosphate
Calcium
Membranes

Keywords

  • Entamoeba histolytica
  • Ins(1,3,4,5)P
  • Ins(1,4,5)P

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Relative importance of inositol (1,4,5)trisphosphate and inositol (1,3,4,5)tetrakisphosphate in Entamoeba histolytica. / Giri, Banabihari; Raha, Sanghamitra; Bhattacharyya, Bhudeb; Biswas, Susweta; Biswas, Birendra B.

In: FEBS Letters, Vol. 393, No. 1, 09.09.1996, p. 109-112.

Research output: Contribution to journalArticle

Giri, Banabihari ; Raha, Sanghamitra ; Bhattacharyya, Bhudeb ; Biswas, Susweta ; Biswas, Birendra B. / Relative importance of inositol (1,4,5)trisphosphate and inositol (1,3,4,5)tetrakisphosphate in Entamoeba histolytica. In: FEBS Letters. 1996 ; Vol. 393, No. 1. pp. 109-112.
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