Remineralization of artificial dentinal caries lesions by biomimetically modified mineral trioxide aggregate

Yi Pin Qi, Nan Li, Li Na Niu, Carolyn M. Primus, Jun Qi Ling, David Henry Pashley, Franklin Chi Meng Tay

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

Fluoride-releasing restorative materials are available for remineralization of enamel and root caries. However, remineralization of dentin is more difficult than remineralization of enamel due to the paucity of apatite seed crystallites along the lesion surface for heterogeneous crystal growth. Extracellular matrix proteins play critical roles in controlling apatite nucleation/growth in collagenous tissues. This study examined the remineralization efficacy of mineral trioxide aggregate (MTA) in phosphate-containing simulated body fluid (SBF) by incorporating polyacrylic acid and sodium tripolyphosphate as biomimetic analogs of matrix proteins for remineralizing caries-like dentin. Artificial caries-like dentin lesions incubated in SBF were remineralized over a 6 week period using MTA alone or MTA containing biomimetic analogs in the absence or presence of dentin adhesive application. Lesion depths and integrated mineral loss were monitored with microcomputed tomography. The ultrastructure of baseline and remineralized lesions was examined by transmission electron microscopy. Dentin remineralization was best achieved using MTA containing biomimetic analogs regardless of whether an adhesive was applied; dentinal tubules within the remineralized dentin were occluded by apatite. It is concluded that the version of MTA employed in this study may be doped with biomimetic analogs for remineralization of unbonded and bonded artificial caries-like lesions in the presence of SBF.

Original languageEnglish (US)
Pages (from-to)836-842
Number of pages7
JournalActa biomaterialia
Volume8
Issue number2
DOIs
StatePublished - Feb 1 2012

Fingerprint

Dentin
Biomimetics
Minerals
Apatites
Body fluids
Apatite
Body Fluids
Enamels
carbopol 940
Dental Enamel
Adhesives
Root Caries
Extracellular Matrix Proteins
Proteins
X-Ray Microtomography
Crystallization
Fluorides
Crystallites
Tomography
Seed

Keywords

  • Biomimetics
  • Caries
  • Micro-computed tomography
  • Mineral trioxide aggregate
  • Tubular occlusion

ASJC Scopus subject areas

  • Biotechnology
  • Biomaterials
  • Biochemistry
  • Biomedical Engineering
  • Molecular Biology

Cite this

Remineralization of artificial dentinal caries lesions by biomimetically modified mineral trioxide aggregate. / Qi, Yi Pin; Li, Nan; Niu, Li Na; Primus, Carolyn M.; Ling, Jun Qi; Pashley, David Henry; Tay, Franklin Chi Meng.

In: Acta biomaterialia, Vol. 8, No. 2, 01.02.2012, p. 836-842.

Research output: Contribution to journalArticle

Qi, Yi Pin ; Li, Nan ; Niu, Li Na ; Primus, Carolyn M. ; Ling, Jun Qi ; Pashley, David Henry ; Tay, Franklin Chi Meng. / Remineralization of artificial dentinal caries lesions by biomimetically modified mineral trioxide aggregate. In: Acta biomaterialia. 2012 ; Vol. 8, No. 2. pp. 836-842.
@article{6e6a25133ba548e3a362bf2303006f0e,
title = "Remineralization of artificial dentinal caries lesions by biomimetically modified mineral trioxide aggregate",
abstract = "Fluoride-releasing restorative materials are available for remineralization of enamel and root caries. However, remineralization of dentin is more difficult than remineralization of enamel due to the paucity of apatite seed crystallites along the lesion surface for heterogeneous crystal growth. Extracellular matrix proteins play critical roles in controlling apatite nucleation/growth in collagenous tissues. This study examined the remineralization efficacy of mineral trioxide aggregate (MTA) in phosphate-containing simulated body fluid (SBF) by incorporating polyacrylic acid and sodium tripolyphosphate as biomimetic analogs of matrix proteins for remineralizing caries-like dentin. Artificial caries-like dentin lesions incubated in SBF were remineralized over a 6 week period using MTA alone or MTA containing biomimetic analogs in the absence or presence of dentin adhesive application. Lesion depths and integrated mineral loss were monitored with microcomputed tomography. The ultrastructure of baseline and remineralized lesions was examined by transmission electron microscopy. Dentin remineralization was best achieved using MTA containing biomimetic analogs regardless of whether an adhesive was applied; dentinal tubules within the remineralized dentin were occluded by apatite. It is concluded that the version of MTA employed in this study may be doped with biomimetic analogs for remineralization of unbonded and bonded artificial caries-like lesions in the presence of SBF.",
keywords = "Biomimetics, Caries, Micro-computed tomography, Mineral trioxide aggregate, Tubular occlusion",
author = "Qi, {Yi Pin} and Nan Li and Niu, {Li Na} and Primus, {Carolyn M.} and Ling, {Jun Qi} and Pashley, {David Henry} and Tay, {Franklin Chi Meng}",
year = "2012",
month = "2",
day = "1",
doi = "10.1016/j.actbio.2011.10.033",
language = "English (US)",
volume = "8",
pages = "836--842",
journal = "Acta Biomaterialia",
issn = "1742-7061",
publisher = "Elsevier BV",
number = "2",

}

TY - JOUR

T1 - Remineralization of artificial dentinal caries lesions by biomimetically modified mineral trioxide aggregate

AU - Qi, Yi Pin

AU - Li, Nan

AU - Niu, Li Na

AU - Primus, Carolyn M.

AU - Ling, Jun Qi

AU - Pashley, David Henry

AU - Tay, Franklin Chi Meng

PY - 2012/2/1

Y1 - 2012/2/1

N2 - Fluoride-releasing restorative materials are available for remineralization of enamel and root caries. However, remineralization of dentin is more difficult than remineralization of enamel due to the paucity of apatite seed crystallites along the lesion surface for heterogeneous crystal growth. Extracellular matrix proteins play critical roles in controlling apatite nucleation/growth in collagenous tissues. This study examined the remineralization efficacy of mineral trioxide aggregate (MTA) in phosphate-containing simulated body fluid (SBF) by incorporating polyacrylic acid and sodium tripolyphosphate as biomimetic analogs of matrix proteins for remineralizing caries-like dentin. Artificial caries-like dentin lesions incubated in SBF were remineralized over a 6 week period using MTA alone or MTA containing biomimetic analogs in the absence or presence of dentin adhesive application. Lesion depths and integrated mineral loss were monitored with microcomputed tomography. The ultrastructure of baseline and remineralized lesions was examined by transmission electron microscopy. Dentin remineralization was best achieved using MTA containing biomimetic analogs regardless of whether an adhesive was applied; dentinal tubules within the remineralized dentin were occluded by apatite. It is concluded that the version of MTA employed in this study may be doped with biomimetic analogs for remineralization of unbonded and bonded artificial caries-like lesions in the presence of SBF.

AB - Fluoride-releasing restorative materials are available for remineralization of enamel and root caries. However, remineralization of dentin is more difficult than remineralization of enamel due to the paucity of apatite seed crystallites along the lesion surface for heterogeneous crystal growth. Extracellular matrix proteins play critical roles in controlling apatite nucleation/growth in collagenous tissues. This study examined the remineralization efficacy of mineral trioxide aggregate (MTA) in phosphate-containing simulated body fluid (SBF) by incorporating polyacrylic acid and sodium tripolyphosphate as biomimetic analogs of matrix proteins for remineralizing caries-like dentin. Artificial caries-like dentin lesions incubated in SBF were remineralized over a 6 week period using MTA alone or MTA containing biomimetic analogs in the absence or presence of dentin adhesive application. Lesion depths and integrated mineral loss were monitored with microcomputed tomography. The ultrastructure of baseline and remineralized lesions was examined by transmission electron microscopy. Dentin remineralization was best achieved using MTA containing biomimetic analogs regardless of whether an adhesive was applied; dentinal tubules within the remineralized dentin were occluded by apatite. It is concluded that the version of MTA employed in this study may be doped with biomimetic analogs for remineralization of unbonded and bonded artificial caries-like lesions in the presence of SBF.

KW - Biomimetics

KW - Caries

KW - Micro-computed tomography

KW - Mineral trioxide aggregate

KW - Tubular occlusion

UR - http://www.scopus.com/inward/record.url?scp=84855427582&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84855427582&partnerID=8YFLogxK

U2 - 10.1016/j.actbio.2011.10.033

DO - 10.1016/j.actbio.2011.10.033

M3 - Article

C2 - 22085925

AN - SCOPUS:84855427582

VL - 8

SP - 836

EP - 842

JO - Acta Biomaterialia

JF - Acta Biomaterialia

SN - 1742-7061

IS - 2

ER -