Renal and glycemic effects of high-dose chromium picolinate in db/db mice

Assessment of DNA damage

Mahmood S Mozaffari, Babak Baban, Rafik A Abdelsayed, Jun Yao Liu, Hereward Wimborne, Nancy Rodriguez, Worku Abebe

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

This study examined renal and glycemic effects of chromium picolinate [Cr(pic)3] supplementation in the context of its purported potential for DNA damage. In preventional protocol, male obese diabetic db/. db mice were fed diets either lacking or containing 5, 10 or 100 mg/kg chromium as Cr(pic)3 from 6 to 24 weeks of age; male lean nondiabetic db/. m mice served as controls. Untreated db/. db mice displayed increased plasma glucose and insulin, hemoglobin A1c, renal tissue advanced glycation end products, albuminuria, glomerular mesangial expansion, urinary 8-hydroxydeoxyguanosine (an index of oxidative DNA damage) and renal tissue immunostaining for γH2AX (a marker of double-strand DNA breaks) compared to db/. m controls. Creatinine clearance was lower in untreated db/. db mice than their db/. m controls, while blood pressure was similar. High Cr(pic)3 intake (i.e., 100-mg/kg diet) mildly improved glycemic status and albuminuria without affecting blood pressure or creatinine clearance. Treatment with Cr(pic)3 did not increase DNA damage despite marked renal accumulation of chromium. In interventional protocol, effects of diets containing 0, 100 and 250 mg/kg supplemental chromium, from 12 to 24 weeks of age, were examined in db/. db mice. The results generally revealed similar effects to those of the 100-mg/kg diet of the preventional protocol. In conclusion, the severely hyperglycemic db/. db mouse displays renal structural and functional abnormalities in association with DNA damage. High-dose Cr(pic)3 treatment mildly improves glycemic control, and it causes moderate reduction in albuminuria, without affecting the histopathological appearance of the kidney and increasing the risk for DNA damage.

Original languageEnglish (US)
Pages (from-to)977-985
Number of pages9
JournalJournal of Nutritional Biochemistry
Volume23
Issue number8
DOIs
StatePublished - Aug 1 2012

Fingerprint

DNA Damage
Nutrition
Kidney
Albuminuria
DNA
Chromium
Diet
Blood pressure
Creatinine
Tissue
Blood Pressure
Advanced Glycosylation End Products
Double-Stranded DNA Breaks
picolinic acid
Hemoglobins
Association reactions
Insulin
Plasmas
Glucose

Keywords

  • γH2AX
  • 8-OHdG
  • Albuminuria
  • Chromium picolinate
  • Diabetes
  • Kidney

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Molecular Biology
  • Nutrition and Dietetics
  • Clinical Biochemistry

Cite this

Renal and glycemic effects of high-dose chromium picolinate in db/db mice : Assessment of DNA damage. / Mozaffari, Mahmood S; Baban, Babak; Abdelsayed, Rafik A; Liu, Jun Yao; Wimborne, Hereward; Rodriguez, Nancy; Abebe, Worku.

In: Journal of Nutritional Biochemistry, Vol. 23, No. 8, 01.08.2012, p. 977-985.

Research output: Contribution to journalArticle

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