Resin-dentin bonds to EDTA-treated vs. acid-etched dentin using ethanol wet-bonding

Salvatore Sauro, Manuel Toledano, Fatima Sánchez Aguilera, Francesco Mannocci, David Henry Pashley, Franklin Chi Meng Tay, Timothy F. Watson, Raquel Osorio

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Objective: To compare resin-dentin bond strengths and the micropermeability of hydrophobic vs. hydrophilic resins bonded to acid-etched or EDTA-treated dentin, using the ethanol wet-bonding technique. Methods: Flat dentin surfaces from extracted human third molars were conditioned before bonding with: 37% H3PO4 (15 s) or 0.1 M EDTA (60 s). Five experimental resin blends of different hydrophilicities and one commercial adhesive (SBMP: Scotchbond Multi-Purpose) were applied to ethanol wet-dentin (1 min) and light-cured (20 s). The solvated resins were used as primers (50% ethanol/50% comonomers) and their respective neat resins were used as the adhesive. The resin-bonded teeth were stored in distilled water (24 h) and sectioned in beams for microtensile bond strength testing. Modes of failure were examined by stereoscopic light microscopy and SEM. Confocal tandem scanning microscopy (TSM) interfacial characterization and micropermeability were also performed after filling the pulp chamber with 1 wt% aqueous rhodamine-B. Results: The most hydrophobic resin 1 gave the lowest bond strength values to acid-etched dentin and all beams failed prematurely when the resin was applied to EDTA-treated dentin. Resins 2 and 3 gave intermediate bond strengths to both conditioned substrates. Resin 4, an acidic hydrophilic resin, gave the highest bond strengths to both EDTA-treated and acid-etched dentin. Resin 5 was the only hydrophilic resin showing poor resin infiltration when applied on acid-etched dentin. Significance: The ethanol wet-bonding technique may improve the infiltration of most of the adhesives used in this study into dentin, especially when applied to EDTA-treated dentin. The chemical composition of the resin blends was a determining factor influencing the ability of adhesives to bond to EDTA-treated or 37% H3PO4 acid-etched dentin, when using the ethanol wet-bonding technique in a clinically relevant time period.

Original languageEnglish (US)
Pages (from-to)368-379
Number of pages12
JournalDental Materials
Volume26
Issue number4
DOIs
StatePublished - Apr 1 2010

Fingerprint

Ethylenediaminetetraacetic acid
Dentin
Edetic Acid
Ethanol
Resins
Acids
Adhesives
rhodamine B
Microscopy
Infiltration
Light
Third Molar
Dental Pulp Cavity
Hydrophobic and Hydrophilic Interactions
Tooth
Hydrophilicity
Polymer blends
Pulp
Optical microscopy

Keywords

  • Confocal microscopy
  • Ethanol-saturated dentin
  • Hydrophobic hybrid layer
  • Micropermeability
  • Microtensile bond strength

ASJC Scopus subject areas

  • Materials Science(all)
  • Dentistry(all)
  • Mechanics of Materials

Cite this

Sauro, S., Toledano, M., Aguilera, F. S., Mannocci, F., Pashley, D. H., Tay, F. C. M., ... Osorio, R. (2010). Resin-dentin bonds to EDTA-treated vs. acid-etched dentin using ethanol wet-bonding. Dental Materials, 26(4), 368-379. https://doi.org/10.1016/j.dental.2009.12.008

Resin-dentin bonds to EDTA-treated vs. acid-etched dentin using ethanol wet-bonding. / Sauro, Salvatore; Toledano, Manuel; Aguilera, Fatima Sánchez; Mannocci, Francesco; Pashley, David Henry; Tay, Franklin Chi Meng; Watson, Timothy F.; Osorio, Raquel.

In: Dental Materials, Vol. 26, No. 4, 01.04.2010, p. 368-379.

Research output: Contribution to journalArticle

Sauro, S, Toledano, M, Aguilera, FS, Mannocci, F, Pashley, DH, Tay, FCM, Watson, TF & Osorio, R 2010, 'Resin-dentin bonds to EDTA-treated vs. acid-etched dentin using ethanol wet-bonding', Dental Materials, vol. 26, no. 4, pp. 368-379. https://doi.org/10.1016/j.dental.2009.12.008
Sauro, Salvatore ; Toledano, Manuel ; Aguilera, Fatima Sánchez ; Mannocci, Francesco ; Pashley, David Henry ; Tay, Franklin Chi Meng ; Watson, Timothy F. ; Osorio, Raquel. / Resin-dentin bonds to EDTA-treated vs. acid-etched dentin using ethanol wet-bonding. In: Dental Materials. 2010 ; Vol. 26, No. 4. pp. 368-379.
@article{3690b7a23dab4c908201bf7a63419772,
title = "Resin-dentin bonds to EDTA-treated vs. acid-etched dentin using ethanol wet-bonding",
abstract = "Objective: To compare resin-dentin bond strengths and the micropermeability of hydrophobic vs. hydrophilic resins bonded to acid-etched or EDTA-treated dentin, using the ethanol wet-bonding technique. Methods: Flat dentin surfaces from extracted human third molars were conditioned before bonding with: 37{\%} H3PO4 (15 s) or 0.1 M EDTA (60 s). Five experimental resin blends of different hydrophilicities and one commercial adhesive (SBMP: Scotchbond Multi-Purpose) were applied to ethanol wet-dentin (1 min) and light-cured (20 s). The solvated resins were used as primers (50{\%} ethanol/50{\%} comonomers) and their respective neat resins were used as the adhesive. The resin-bonded teeth were stored in distilled water (24 h) and sectioned in beams for microtensile bond strength testing. Modes of failure were examined by stereoscopic light microscopy and SEM. Confocal tandem scanning microscopy (TSM) interfacial characterization and micropermeability were also performed after filling the pulp chamber with 1 wt{\%} aqueous rhodamine-B. Results: The most hydrophobic resin 1 gave the lowest bond strength values to acid-etched dentin and all beams failed prematurely when the resin was applied to EDTA-treated dentin. Resins 2 and 3 gave intermediate bond strengths to both conditioned substrates. Resin 4, an acidic hydrophilic resin, gave the highest bond strengths to both EDTA-treated and acid-etched dentin. Resin 5 was the only hydrophilic resin showing poor resin infiltration when applied on acid-etched dentin. Significance: The ethanol wet-bonding technique may improve the infiltration of most of the adhesives used in this study into dentin, especially when applied to EDTA-treated dentin. The chemical composition of the resin blends was a determining factor influencing the ability of adhesives to bond to EDTA-treated or 37{\%} H3PO4 acid-etched dentin, when using the ethanol wet-bonding technique in a clinically relevant time period.",
keywords = "Confocal microscopy, Ethanol-saturated dentin, Hydrophobic hybrid layer, Micropermeability, Microtensile bond strength",
author = "Salvatore Sauro and Manuel Toledano and Aguilera, {Fatima S{\'a}nchez} and Francesco Mannocci and Pashley, {David Henry} and Tay, {Franklin Chi Meng} and Watson, {Timothy F.} and Raquel Osorio",
year = "2010",
month = "4",
day = "1",
doi = "10.1016/j.dental.2009.12.008",
language = "English (US)",
volume = "26",
pages = "368--379",
journal = "Dental Materials",
issn = "0109-5641",
publisher = "Elsevier Science",
number = "4",

}

TY - JOUR

T1 - Resin-dentin bonds to EDTA-treated vs. acid-etched dentin using ethanol wet-bonding

AU - Sauro, Salvatore

AU - Toledano, Manuel

AU - Aguilera, Fatima Sánchez

AU - Mannocci, Francesco

AU - Pashley, David Henry

AU - Tay, Franklin Chi Meng

AU - Watson, Timothy F.

AU - Osorio, Raquel

PY - 2010/4/1

Y1 - 2010/4/1

N2 - Objective: To compare resin-dentin bond strengths and the micropermeability of hydrophobic vs. hydrophilic resins bonded to acid-etched or EDTA-treated dentin, using the ethanol wet-bonding technique. Methods: Flat dentin surfaces from extracted human third molars were conditioned before bonding with: 37% H3PO4 (15 s) or 0.1 M EDTA (60 s). Five experimental resin blends of different hydrophilicities and one commercial adhesive (SBMP: Scotchbond Multi-Purpose) were applied to ethanol wet-dentin (1 min) and light-cured (20 s). The solvated resins were used as primers (50% ethanol/50% comonomers) and their respective neat resins were used as the adhesive. The resin-bonded teeth were stored in distilled water (24 h) and sectioned in beams for microtensile bond strength testing. Modes of failure were examined by stereoscopic light microscopy and SEM. Confocal tandem scanning microscopy (TSM) interfacial characterization and micropermeability were also performed after filling the pulp chamber with 1 wt% aqueous rhodamine-B. Results: The most hydrophobic resin 1 gave the lowest bond strength values to acid-etched dentin and all beams failed prematurely when the resin was applied to EDTA-treated dentin. Resins 2 and 3 gave intermediate bond strengths to both conditioned substrates. Resin 4, an acidic hydrophilic resin, gave the highest bond strengths to both EDTA-treated and acid-etched dentin. Resin 5 was the only hydrophilic resin showing poor resin infiltration when applied on acid-etched dentin. Significance: The ethanol wet-bonding technique may improve the infiltration of most of the adhesives used in this study into dentin, especially when applied to EDTA-treated dentin. The chemical composition of the resin blends was a determining factor influencing the ability of adhesives to bond to EDTA-treated or 37% H3PO4 acid-etched dentin, when using the ethanol wet-bonding technique in a clinically relevant time period.

AB - Objective: To compare resin-dentin bond strengths and the micropermeability of hydrophobic vs. hydrophilic resins bonded to acid-etched or EDTA-treated dentin, using the ethanol wet-bonding technique. Methods: Flat dentin surfaces from extracted human third molars were conditioned before bonding with: 37% H3PO4 (15 s) or 0.1 M EDTA (60 s). Five experimental resin blends of different hydrophilicities and one commercial adhesive (SBMP: Scotchbond Multi-Purpose) were applied to ethanol wet-dentin (1 min) and light-cured (20 s). The solvated resins were used as primers (50% ethanol/50% comonomers) and their respective neat resins were used as the adhesive. The resin-bonded teeth were stored in distilled water (24 h) and sectioned in beams for microtensile bond strength testing. Modes of failure were examined by stereoscopic light microscopy and SEM. Confocal tandem scanning microscopy (TSM) interfacial characterization and micropermeability were also performed after filling the pulp chamber with 1 wt% aqueous rhodamine-B. Results: The most hydrophobic resin 1 gave the lowest bond strength values to acid-etched dentin and all beams failed prematurely when the resin was applied to EDTA-treated dentin. Resins 2 and 3 gave intermediate bond strengths to both conditioned substrates. Resin 4, an acidic hydrophilic resin, gave the highest bond strengths to both EDTA-treated and acid-etched dentin. Resin 5 was the only hydrophilic resin showing poor resin infiltration when applied on acid-etched dentin. Significance: The ethanol wet-bonding technique may improve the infiltration of most of the adhesives used in this study into dentin, especially when applied to EDTA-treated dentin. The chemical composition of the resin blends was a determining factor influencing the ability of adhesives to bond to EDTA-treated or 37% H3PO4 acid-etched dentin, when using the ethanol wet-bonding technique in a clinically relevant time period.

KW - Confocal microscopy

KW - Ethanol-saturated dentin

KW - Hydrophobic hybrid layer

KW - Micropermeability

KW - Microtensile bond strength

UR - http://www.scopus.com/inward/record.url?scp=76849098480&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=76849098480&partnerID=8YFLogxK

U2 - 10.1016/j.dental.2009.12.008

DO - 10.1016/j.dental.2009.12.008

M3 - Article

VL - 26

SP - 368

EP - 379

JO - Dental Materials

JF - Dental Materials

SN - 0109-5641

IS - 4

ER -