Response of human artery, vein, and cultured smooth muscle cells to atrial and C-type natriuretic peptides

Li Ming Zhang, Manuel R Castresana, Miles H. McDonald, Joseph H. Johnson, Walter H. Newman

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Objectives: We determined the response of intracellular cyclic GMP in human arteries and veins and in smooth muscle cells cultured from these vessels to C-type natriuretic peptide in comparison with atrial natriuretic peptide. Design: Repeated-measures analysis of concentration-response curves. Setting: Anesthesia research laboratory. Subjects: Vascular smooth muscle cells from human blood vessels obtained with Institutional Review Board approval and patient consent. Measurements and Main Results: Segments of internal mammary artery and saphenous vein were obtained from patients undergoing coronary artery bypass surgery. Smooth muscle cells were cultured from these vessels. Concentration-response curves of intracellular cyclic GMP were determined and analyzed by two-way analysis of variance with repeated measures. In segments of intact saphenous vein, C-type natriuretic peptide was significantly more effective than atrial natriuretic peptide (10-fold increase in cyclic GMP in response to 1 μM of C-type natriuretic peptide vs. six-fold increase in cyclic GMP in response to 1 μM of atrial natriuretic peptide, p < .05). In rings of intact internal mammary artery, 1 μM of atrial natriuretic peptide (26-fold increase in cyclic GMP over basal value) was more effective than 1 μM of C-type natriuretic peptide (three-fold increase in cyclic GMP over basal value, p < .05). In cultured cells from these vessels, the pattern of response to C-type natriuretic peptide and atrial natriuretic peptide was the same as in the intact vessels. Conclusions: These results indicated that human smooth muscle cells in arteries and veins express both forms of natriuretic peptide receptors but that atrial natriuretic peptide acts primarily on the artery and C-type natriuretic peptide acts predominately on the vein. Increased concentrations of C-type natriuretic peptide could contribute to venous pooling in septic shock.

Original languageEnglish (US)
Pages (from-to)306-310
Number of pages5
JournalCritical Care Medicine
Volume24
Issue number2
DOIs
StatePublished - Feb 1 1996
Externally publishedYes

Fingerprint

C-Type Natriuretic Peptide
Atrial Natriuretic Factor
Cyclic GMP
Smooth Muscle Myocytes
Veins
Arteries
Atrial Natriuretic Factor Receptors
Mammary Arteries
Saphenous Vein
Research Ethics Committees
Septic Shock
Vascular Smooth Muscle
Coronary Artery Bypass
Blood Vessels
Cultured Cells
Analysis of Variance
Anesthesia

Keywords

  • atrial natriuretic peptide
  • blood vessels
  • guanosine cyclic 3'5'-monophosphate
  • receptors
  • septic shock
  • vascular smooth muscle cells

ASJC Scopus subject areas

  • Critical Care and Intensive Care Medicine

Cite this

Response of human artery, vein, and cultured smooth muscle cells to atrial and C-type natriuretic peptides. / Zhang, Li Ming; Castresana, Manuel R; McDonald, Miles H.; Johnson, Joseph H.; Newman, Walter H.

In: Critical Care Medicine, Vol. 24, No. 2, 01.02.1996, p. 306-310.

Research output: Contribution to journalArticle

Zhang, Li Ming ; Castresana, Manuel R ; McDonald, Miles H. ; Johnson, Joseph H. ; Newman, Walter H. / Response of human artery, vein, and cultured smooth muscle cells to atrial and C-type natriuretic peptides. In: Critical Care Medicine. 1996 ; Vol. 24, No. 2. pp. 306-310.
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AU - Johnson, Joseph H.

AU - Newman, Walter H.

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AB - Objectives: We determined the response of intracellular cyclic GMP in human arteries and veins and in smooth muscle cells cultured from these vessels to C-type natriuretic peptide in comparison with atrial natriuretic peptide. Design: Repeated-measures analysis of concentration-response curves. Setting: Anesthesia research laboratory. Subjects: Vascular smooth muscle cells from human blood vessels obtained with Institutional Review Board approval and patient consent. Measurements and Main Results: Segments of internal mammary artery and saphenous vein were obtained from patients undergoing coronary artery bypass surgery. Smooth muscle cells were cultured from these vessels. Concentration-response curves of intracellular cyclic GMP were determined and analyzed by two-way analysis of variance with repeated measures. In segments of intact saphenous vein, C-type natriuretic peptide was significantly more effective than atrial natriuretic peptide (10-fold increase in cyclic GMP in response to 1 μM of C-type natriuretic peptide vs. six-fold increase in cyclic GMP in response to 1 μM of atrial natriuretic peptide, p < .05). In rings of intact internal mammary artery, 1 μM of atrial natriuretic peptide (26-fold increase in cyclic GMP over basal value) was more effective than 1 μM of C-type natriuretic peptide (three-fold increase in cyclic GMP over basal value, p < .05). In cultured cells from these vessels, the pattern of response to C-type natriuretic peptide and atrial natriuretic peptide was the same as in the intact vessels. Conclusions: These results indicated that human smooth muscle cells in arteries and veins express both forms of natriuretic peptide receptors but that atrial natriuretic peptide acts primarily on the artery and C-type natriuretic peptide acts predominately on the vein. Increased concentrations of C-type natriuretic peptide could contribute to venous pooling in septic shock.

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KW - blood vessels

KW - guanosine cyclic 3'5'-monophosphate

KW - receptors

KW - septic shock

KW - vascular smooth muscle cells

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